Identification and quantitation of unsaturated fatty acid isomers by electrospray ionization tandem mass spectrometry: A shotgun lipidomics approach

Kui Yang, Zhongdan Zhao, Richard W. Gross, Xianlin Han

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Identification and quantification of unsaturated fatty acid (FA) isomers in a biological system are significant in the study of lipid metabolism and catabolism, membrane biophysics, and pathogenesis of diseases but are challenging in lipidomics. We developed a novel approach for identification and quantitation of unsaturated FA isomers by exploiting two facts: (1) unsaturated FA anions yield fragment ion(s) from loss of CO 2 or H 2O from the anions upon collision-induced dissociation; and (2) the fragment ions yielded from discrete FA isomers have distinct profiles of the fragment ion intensity vs. collision conditions. These distinct profiles likely result from the differential interactions of the negative charge of the fragment ion with the electron clouds of the double bonds due to their different distances in discrete FA isomers. The novel approach was also extended to analyze the double bond isomers of FA chains present in phospholipids by multistage tandem mass spectrometry. Collectively, we developed a new approach for identification and quantification of the double bond isomers of endogenous FA species or FA chains present in intact phospholipid species. We believe that this approach should further advance the lipidomic power for identification of the biochemical mechanisms underlying metabolic diseases.

Original languageEnglish (US)
Pages (from-to)4243-4250
Number of pages8
JournalAnalytical Chemistry
Volume83
Issue number11
DOIs
StatePublished - Jun 1 2011
Externally publishedYes

Fingerprint

Electrospray ionization
Unsaturated Fatty Acids
Isomers
Mass spectrometry
Fatty Acids
Ions
Anions
Phospholipids
Biophysics
Biological systems
Carbon Monoxide
Membranes
Electrons

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Identification and quantitation of unsaturated fatty acid isomers by electrospray ionization tandem mass spectrometry : A shotgun lipidomics approach. / Yang, Kui; Zhao, Zhongdan; Gross, Richard W.; Han, Xianlin.

In: Analytical Chemistry, Vol. 83, No. 11, 01.06.2011, p. 4243-4250.

Research output: Contribution to journalArticle

@article{9fbda3c1942647a2aee08d78c45d7cf0,
title = "Identification and quantitation of unsaturated fatty acid isomers by electrospray ionization tandem mass spectrometry: A shotgun lipidomics approach",
abstract = "Identification and quantification of unsaturated fatty acid (FA) isomers in a biological system are significant in the study of lipid metabolism and catabolism, membrane biophysics, and pathogenesis of diseases but are challenging in lipidomics. We developed a novel approach for identification and quantitation of unsaturated FA isomers by exploiting two facts: (1) unsaturated FA anions yield fragment ion(s) from loss of CO 2 or H 2O from the anions upon collision-induced dissociation; and (2) the fragment ions yielded from discrete FA isomers have distinct profiles of the fragment ion intensity vs. collision conditions. These distinct profiles likely result from the differential interactions of the negative charge of the fragment ion with the electron clouds of the double bonds due to their different distances in discrete FA isomers. The novel approach was also extended to analyze the double bond isomers of FA chains present in phospholipids by multistage tandem mass spectrometry. Collectively, we developed a new approach for identification and quantification of the double bond isomers of endogenous FA species or FA chains present in intact phospholipid species. We believe that this approach should further advance the lipidomic power for identification of the biochemical mechanisms underlying metabolic diseases.",
author = "Kui Yang and Zhongdan Zhao and Gross, {Richard W.} and Xianlin Han",
year = "2011",
month = "6",
day = "1",
doi = "10.1021/ac2006119",
language = "English (US)",
volume = "83",
pages = "4243--4250",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "11",

}

TY - JOUR

T1 - Identification and quantitation of unsaturated fatty acid isomers by electrospray ionization tandem mass spectrometry

T2 - A shotgun lipidomics approach

AU - Yang, Kui

AU - Zhao, Zhongdan

AU - Gross, Richard W.

AU - Han, Xianlin

PY - 2011/6/1

Y1 - 2011/6/1

N2 - Identification and quantification of unsaturated fatty acid (FA) isomers in a biological system are significant in the study of lipid metabolism and catabolism, membrane biophysics, and pathogenesis of diseases but are challenging in lipidomics. We developed a novel approach for identification and quantitation of unsaturated FA isomers by exploiting two facts: (1) unsaturated FA anions yield fragment ion(s) from loss of CO 2 or H 2O from the anions upon collision-induced dissociation; and (2) the fragment ions yielded from discrete FA isomers have distinct profiles of the fragment ion intensity vs. collision conditions. These distinct profiles likely result from the differential interactions of the negative charge of the fragment ion with the electron clouds of the double bonds due to their different distances in discrete FA isomers. The novel approach was also extended to analyze the double bond isomers of FA chains present in phospholipids by multistage tandem mass spectrometry. Collectively, we developed a new approach for identification and quantification of the double bond isomers of endogenous FA species or FA chains present in intact phospholipid species. We believe that this approach should further advance the lipidomic power for identification of the biochemical mechanisms underlying metabolic diseases.

AB - Identification and quantification of unsaturated fatty acid (FA) isomers in a biological system are significant in the study of lipid metabolism and catabolism, membrane biophysics, and pathogenesis of diseases but are challenging in lipidomics. We developed a novel approach for identification and quantitation of unsaturated FA isomers by exploiting two facts: (1) unsaturated FA anions yield fragment ion(s) from loss of CO 2 or H 2O from the anions upon collision-induced dissociation; and (2) the fragment ions yielded from discrete FA isomers have distinct profiles of the fragment ion intensity vs. collision conditions. These distinct profiles likely result from the differential interactions of the negative charge of the fragment ion with the electron clouds of the double bonds due to their different distances in discrete FA isomers. The novel approach was also extended to analyze the double bond isomers of FA chains present in phospholipids by multistage tandem mass spectrometry. Collectively, we developed a new approach for identification and quantification of the double bond isomers of endogenous FA species or FA chains present in intact phospholipid species. We believe that this approach should further advance the lipidomic power for identification of the biochemical mechanisms underlying metabolic diseases.

UR - http://www.scopus.com/inward/record.url?scp=79957953905&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79957953905&partnerID=8YFLogxK

U2 - 10.1021/ac2006119

DO - 10.1021/ac2006119

M3 - Article

C2 - 21500847

AN - SCOPUS:79957953905

VL - 83

SP - 4243

EP - 4250

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 11

ER -