Identification and partial characterization of a nucleolar antigen with a molecular weight of 145,000 found in a broad range of human cancers

James W Freeman, D. K. McRorie, R. K. Busch, F. Gyorkey, P. Gyorkey, B. E. Ross, W. H. Spohn

Research output: Contribution to journalArticle

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Abstract

Previous studies in our laboratory have indicated the presence of nucleolar antigens in tumors which were not detected in normal tissues. Some of the polyclonal antisera produced in these studies were shown to identify a M(r) 145,000 nucleolar antigen on immunoblots of tumor nucleoli but not in normal human liver nucleoli. A monoclonal antibody to a M(r) 145,000 nucleolar protein (p145) was produced by immunization of mice with a nucleolar extract of HeLa cells which is enriched with this antigen. The monoclonal antibody showed bright nucleolar immunofluorescence localization in a broad range of human tumors including cancers of the gastrointestinal tract, genitourinary tract, lung, liver, muscle, cartilage, and blood. The p145 nucleolar antigen was not detected in most normal human tissues or in benign tumors, with only weak nucleolar staining observed in spermatogonia of the testes and in ductal regions of some hypertrophied prostates. Nucleolar antigen p145 was extracted from HeLa cell nculeoli by homogenization in a 0.01 M Tris buffer containing 0.2% deoxycholate. On sucrose density gradient centrifugation, the antigen remained sedimented with the nucleolar ribonucleoprotein fraction. Nucleolar antigen p145 was released from ribonucleoproteins following treatment with 4 M guanidinium hydrochloride or RNase. Peptide mapping of nucleolar antigen p145 showed that it was distinct from other known nucleolar antigens. Although it remains to be determined if the p145 antigen plays a role in cell transformation, maintenance of the malignant phenotype, or in cell division, it may have value as a tumor marker or as a therapeutic target.

Original languageEnglish (US)
Pages (from-to)3593-3598
Number of pages6
JournalCancer Research
Volume46
Issue number7
StatePublished - 1986
Externally publishedYes

Fingerprint

Molecular Weight
Antigens
Ribonucleoproteins
Neoplasms
HeLa Cells
Monoclonal Antibodies
Spermatogonia
Tromethamine
Peptide Mapping
Gastrointestinal Neoplasms
Deoxycholic Acid
Density Gradient Centrifugation
Liver
Guanidine
Neoplasm Antigens
Tumor Biomarkers
Ribonucleases
Cell Division
Cartilage
Fluorescent Antibody Technique

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Freeman, J. W., McRorie, D. K., Busch, R. K., Gyorkey, F., Gyorkey, P., Ross, B. E., & Spohn, W. H. (1986). Identification and partial characterization of a nucleolar antigen with a molecular weight of 145,000 found in a broad range of human cancers. Cancer Research, 46(7), 3593-3598.

Identification and partial characterization of a nucleolar antigen with a molecular weight of 145,000 found in a broad range of human cancers. / Freeman, James W; McRorie, D. K.; Busch, R. K.; Gyorkey, F.; Gyorkey, P.; Ross, B. E.; Spohn, W. H.

In: Cancer Research, Vol. 46, No. 7, 1986, p. 3593-3598.

Research output: Contribution to journalArticle

Freeman, JW, McRorie, DK, Busch, RK, Gyorkey, F, Gyorkey, P, Ross, BE & Spohn, WH 1986, 'Identification and partial characterization of a nucleolar antigen with a molecular weight of 145,000 found in a broad range of human cancers', Cancer Research, vol. 46, no. 7, pp. 3593-3598.
Freeman, James W ; McRorie, D. K. ; Busch, R. K. ; Gyorkey, F. ; Gyorkey, P. ; Ross, B. E. ; Spohn, W. H. / Identification and partial characterization of a nucleolar antigen with a molecular weight of 145,000 found in a broad range of human cancers. In: Cancer Research. 1986 ; Vol. 46, No. 7. pp. 3593-3598.
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