Identification and Partial Characterization of a Mr40, 000 Nucleolar Antigen Associated with Cell Proliferation

Amitava Chatterjee, James W. Freeman, Harris Busch

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44 Scopus citations

Abstract

The present study reports the identification and partial characterization of a novel Mr 40, 000 nucleolar antigen (P40) by monoclonal antibodies. Monoclonal antibodies to this protein were obtained when a nucleolar protein extract separated from the immunodominant protein C23 was used to immunize BALB/c mice; 12 hybridoma clones produced antibodies to this protein. P40 was not detected in normal human kidney, liver, and leukocytes but was readily demonstrable in a variety of human malignant tissues. This newly identified P40 antigen differs in its specific nucleolar localization from cyclin (proliferating cell nuclear antigen), a Mr 35, 000 antigen which is largely in the nucleoplasm. In addition, cyclin appears in the nucleolus in S-phase; P40 appears in the nucleolus 6 h after refeeding serum-starved HeLa cells.

Original languageEnglish (US)
Pages (from-to)1123-1129
Number of pages7
JournalCancer Research
Volume47
Issue number4
StatePublished - Feb 1987

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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