Abstract
The present study reports the identification and partial characterization of a novel Mr 40, 000 nucleolar antigen (P40) by monoclonal antibodies. Monoclonal antibodies to this protein were obtained when a nucleolar protein extract separated from the immunodominant protein C23 was used to immunize BALB/c mice; 12 hybridoma clones produced antibodies to this protein. P40 was not detected in normal human kidney, liver, and leukocytes but was readily demonstrable in a variety of human malignant tissues. This newly identified P40 antigen differs in its specific nucleolar localization from cyclin (proliferating cell nuclear antigen), a Mr 35, 000 antigen which is largely in the nucleoplasm. In addition, cyclin appears in the nucleolus in S-phase; P40 appears in the nucleolus 6 h after refeeding serum-starved HeLa cells.
Original language | English (US) |
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Pages (from-to) | 1123-1129 |
Number of pages | 7 |
Journal | Cancer Research |
Volume | 47 |
Issue number | 4 |
State | Published - Feb 1987 |
ASJC Scopus subject areas
- Oncology
- Cancer Research