Identification and interpretation of complexity in sedimentation velocity boundaries

Borries Demeler, Hashim Saber, Jeffrey C. Hansen

Research output: Contribution to journalArticle

137 Scopus citations

Abstract

Synthetic sedimentation velocity boundaries were generated using finite- element solutions to the original and modified forms of the Lamm equation. Situations modeled included ideal single- and multicomponent samples, concentration-dependent samples, noninteracting multicomponent samples, and reversibly self-associating samples. Synthetic boundaries subsequently were analyzed using the method of van Halde and Weischet, and results were compared against known input parameters. Results indicate that this analytical method provides rigorous diagnostics for virtually every type of sample complexity encountered experimentally. Accordingly, both the power and utility of sedimentation velocity experiments have been significantly expanded.

Original languageEnglish (US)
Pages (from-to)397-407
Number of pages11
JournalBiophysical Journal
Volume72
Issue number1
DOIs
StatePublished - Jan 1997

ASJC Scopus subject areas

  • Biophysics

Fingerprint Dive into the research topics of 'Identification and interpretation of complexity in sedimentation velocity boundaries'. Together they form a unique fingerprint.

Cite this