Identification and characterization of midgut proteases in Achaea janata and their implications

Madhusudhan Budatha, Gargi Meur, Aparna Dutta-Gupta

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Insect midgut proteases are excellent targets for insecticidal agents such as Bacillus thuringiensis Cry toxins and protease inhibitors. The midgut proteases of Achaea janata have been characterized and Casein zymograms indicated at least five distinct activities corresponding to approx 17, 20, 29 and 80, and 90 kDa. Using a combination of synthetic substrates and specific inhibitors in casein zymograms, photometric assays and activity blots, three trypsin-like and one elastase-like serine proteases were identified but no chymotrypsin-like activity. Various proteinase inhibitors displayed differential inhibitory effects towards the midgut proteases.

Original languageEnglish (US)
Pages (from-to)305-310
Number of pages6
JournalBiotechnology Letters
Volume30
Issue number2
DOIs
StatePublished - Feb 1 2008
Externally publishedYes

Keywords

  • Aminopeptidase
  • Bacillus thuringiensis
  • Chymotrypsin
  • Cry toxin
  • Elastase
  • GPI anchor
  • Proteases
  • Trypsin
  • Zymogram

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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