Identification and characterization of a surface-associated, subtilisin-like serine protease in Trichomonas vaginalis

Pablo Hernández-Romano, Roberto Hernández, Rossana Arroyo, John F. Alderete, Imelda López-Villaseñor

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Trichomonas vaginalis is a protozoan parasite causing trichomonosis, a sexually transmitted infection in humans. This parasite has numerous proteases, most of which are cysteine proteases that appear to be involved in adherence and cytotoxicity of host cells. In this report we identify and characterize a putative subtilisin-like serine protease (SUB1). The sub1 gene encodes a 101-kDa protein. In silico analyses predict signal and pro-peptides at the N-terminus, and a transmembrane helix at the carboxy-terminal region. The sub1 gene was found as single copy by Southern analysis, albeit additional serine protease related genes are annotated in the T. vaginalis genome. The expression of sub1 could only be detected by RT-PCR and Ribonuclease Protection Assays, suggesting a low abundant mRNA. The sub1 gene transcription start site was correctly assigned by RPA. The transcript abundance was found to be modulated by the availability of iron in the growth medium. Antibodies raised to a specific SUB1 peptide recognized a single protein band (∼82 kDa) in Western blots, possibly representing the mature form of the protein. Immunofluorescence showed SUB1 on the trichomonad surface, and in dispersed vesicles throughout the cytoplasm. A bioinformatic analysis of genes annotated as serine proteases in the T. vaginalis genome is also presented. To our knowledge this is the first putative serine protease experimentally described for T. vaginalis.

Original languageEnglish (US)
Pages (from-to)1621-1635
Number of pages15
JournalParasitology
Volume137
Issue number11
DOIs
StatePublished - Sep 2010
Externally publishedYes

Keywords

  • cellular localization
  • serine protease
  • subtilisin
  • surface protein
  • Trichomonas vaginalis

ASJC Scopus subject areas

  • Parasitology
  • Animal Science and Zoology
  • Infectious Diseases

Fingerprint

Dive into the research topics of 'Identification and characterization of a surface-associated, subtilisin-like serine protease in Trichomonas vaginalis'. Together they form a unique fingerprint.

Cite this