Hypoxic neuronal injury in vitro depends on extracellular glutamine

Mark P. Goldberg; Hannelore Monyer; Dennis W. Choi

doi:10.1016/0304-3940(88)90269-8

Hypoxic neuronal injury in vitro depends on extracellular glutamine

Mark P. Goldberg, Hannelore Monyer, Dennis W. Choi

Research output: Contribution to journal › Article › peer-review

61 Scopus citations

Abstract

Hypoxic neuronal injury (HNI) in cortical cell cultures was enhanced in a concentration-dependent fashion by the presence of 500 μM to 2 mM (EC₅₀ about 500 μM) glutamine in the medium, concentrations approximating those normally present in cerebrospinal fluid (CSF). Regardless of the glutamine concentration, glutamate receptor antagonists 2-amino-5-phosphonovalerate or dextrorphan could substantially reduce HNI. Thus, the availability of extracellular glutamine could be a determinant of hypoxic neuronal injury in vivo, most likely reflecting its importance in the synthesis of the neurotransmitter excitotoxins glutamate and aspartate.

Original language	English (US)
Pages (from-to)	52-57
Number of pages	6
Journal	Neuroscience Letters
Volume	94
Issue number	1-2
DOIs	https://doi.org/10.1016/0304-3940(88)90269-8
State	Published - Nov 22 1988
Externally published	Yes

Keywords

Aspartate
Cell culture
Glutamate
Glutamine
Hypoxia
Neurotoxicity

ASJC Scopus subject areas

General Neuroscience

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10.1016/0304-3940(88)90269-8

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title = "Hypoxic neuronal injury in vitro depends on extracellular glutamine",

abstract = "Hypoxic neuronal injury (HNI) in cortical cell cultures was enhanced in a concentration-dependent fashion by the presence of 500 μM to 2 mM (EC50 about 500 μM) glutamine in the medium, concentrations approximating those normally present in cerebrospinal fluid (CSF). Regardless of the glutamine concentration, glutamate receptor antagonists 2-amino-5-phosphonovalerate or dextrorphan could substantially reduce HNI. Thus, the availability of extracellular glutamine could be a determinant of hypoxic neuronal injury in vivo, most likely reflecting its importance in the synthesis of the neurotransmitter excitotoxins glutamate and aspartate.",

keywords = "Aspartate, Cell culture, Glutamate, Glutamine, Hypoxia, Neurotoxicity",

author = "Goldberg, {Mark P.} and Hannelore Monyer and Choi, {Dennis W.}",

note = "Funding Information: We thank V. Viseskul for experta ssistancwe ith cell culturesa, nd S. Peroutkaf or use of HPLC equipmentT. his researchw as supportedb y NIH Grant NS12151,a nd by a grant from the HereditaryD iseaseF oundationP. ost-doctorafle llowships up-port was provided by NIH Epilepsy Training Grant NS07820 (M.P.G.) and the DeutscheF orschungsgemeinsch(aHf.tM .).",

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doi = "10.1016/0304-3940(88)90269-8",

language = "English (US)",

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journal = "Neuroscience Letters",

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T1 - Hypoxic neuronal injury in vitro depends on extracellular glutamine

AU - Goldberg, Mark P.

AU - Monyer, Hannelore

AU - Choi, Dennis W.

N1 - Funding Information: We thank V. Viseskul for experta ssistancwe ith cell culturesa, nd S. Peroutkaf or use of HPLC equipmentT. his researchw as supportedb y NIH Grant NS12151,a nd by a grant from the HereditaryD iseaseF oundationP. ost-doctorafle llowships up-port was provided by NIH Epilepsy Training Grant NS07820 (M.P.G.) and the DeutscheF orschungsgemeinsch(aHf.tM .).

PY - 1988/11/22

Y1 - 1988/11/22

N2 - Hypoxic neuronal injury (HNI) in cortical cell cultures was enhanced in a concentration-dependent fashion by the presence of 500 μM to 2 mM (EC50 about 500 μM) glutamine in the medium, concentrations approximating those normally present in cerebrospinal fluid (CSF). Regardless of the glutamine concentration, glutamate receptor antagonists 2-amino-5-phosphonovalerate or dextrorphan could substantially reduce HNI. Thus, the availability of extracellular glutamine could be a determinant of hypoxic neuronal injury in vivo, most likely reflecting its importance in the synthesis of the neurotransmitter excitotoxins glutamate and aspartate.

AB - Hypoxic neuronal injury (HNI) in cortical cell cultures was enhanced in a concentration-dependent fashion by the presence of 500 μM to 2 mM (EC50 about 500 μM) glutamine in the medium, concentrations approximating those normally present in cerebrospinal fluid (CSF). Regardless of the glutamine concentration, glutamate receptor antagonists 2-amino-5-phosphonovalerate or dextrorphan could substantially reduce HNI. Thus, the availability of extracellular glutamine could be a determinant of hypoxic neuronal injury in vivo, most likely reflecting its importance in the synthesis of the neurotransmitter excitotoxins glutamate and aspartate.

KW - Aspartate

KW - Cell culture

KW - Glutamate

KW - Glutamine

KW - Hypoxia

KW - Neurotoxicity

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U2 - 10.1016/0304-3940(88)90269-8

DO - 10.1016/0304-3940(88)90269-8

M3 - Article

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AN - SCOPUS:0023700925

SN - 0304-3940

VL - 94

SP - 52

EP - 57

JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 1-2

ER -

Hypoxic neuronal injury in vitro depends on extracellular glutamine

Abstract

Keywords

ASJC Scopus subject areas

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