Hypoperfusion causes increased production of interleukin 6 and tumor necrosis factor α in the isolated, dually perfused placental cotyledon

Brian T. Pierce, Lisa M. Pierce, Richard K. Wagner, Christina C. Apodaca, Roderick F. Hume, Peter E. Nielsen, Byron C. Calhoun

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

OBJECTIVE: Our purpose was to determine whether exposure of the isolated, perfused human placental cotyledon to different fetal circuit perfusion rates, and to concomitant pressure differences, alters placental production of interleukin 6 and tumor necrosis factor α. STUDY DESIGN: The maternal and fetal circulations of 2 cotyledons from 5 placentas were perfused for 4 hours. The fetal circulation of 1 cotyledon was perfused at a low rate of 1 mL/min, and the other at a high rate of 10 mL/min. The maternal circulation of each cotyledon was perfused at 10 mL/min. Effluents from the fetal circulation were collected at hourly intervals, and concentrations of interleukin 6 and tumor necrosis factor α were determined by enzyme-linked immunosorbent assay. Concentrations of interleukin 6, obtained from a prior study with an estimated physiologic fetal circulation rate of 4 mL/min, were compared with the low and high perfusion rate results. RESULTS: Concentrations of interleukin 6 and tumor necrosis factor α were greater at the perfusion rate of 1 mL/min, in comparison with the perfusion rate of 10 mL/min, with statistically significant differences achieved at 2 and 4 hours for interleukin 6 and at 4 hours for tumor necrosis factor α. Concentrations of both cytokines increased exponentially with time. Placental perfusion pressures were significantly greater at the perfusion rate of 10 mL/min. CONCLUSION: Placental hypoperfusion results in an increased production of both interleukin 6 and tumor necrosis factor α. This finding links placental perfusion abnormalities to the myriad of disorders associated with elevated concentrations of inflammatory cytokines, including cerebral palsy.

Original languageEnglish (US)
Pages (from-to)863-867
Number of pages5
JournalAmerican Journal of Obstetrics and Gynecology
Volume183
Issue number4
DOIs
StatePublished - 2000
Externally publishedYes

Keywords

  • Cerebral palsy
  • Cytokines
  • Fetal inflammatory response syndrome
  • Interleukin 6
  • Placenta perfusion
  • Tumor necrosis factor α

ASJC Scopus subject areas

  • Obstetrics and Gynecology

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