Hypoenergetic high-carbohydrate or high-fat parenteral nutrition induces a similar metabolic with response with differential effects on hepatic IGF-I mRNA in dexamethasone-treated rats

Karen R. Kritsch, Sangita Murali, Martin L. Adamo, Murray K. Clayton, Denise M. Ney

Research output: Contribution to journalArticle

3 Scopus citations


The optimal level of energy for critically ill patients who require parenteral nutrition (PN) is unclear. Our objective was to determine whether 50% energy (50%E) restriction due to a reduction in carbohydrate or fat, with provision of adequate protein and micronutrients, ameliorates the detrimental effects of dexamethasone (Dex) on body protein catabolism, insulin resistance, and insulin-like growth factor-I (IGF-I) responses in rats administered PN. The experiment included 6 PN groups, adequate energy (AE) ± Dex, 50% AE with high carbohydrate (50%E ↑ CHO) ± Dex and 50% AE with high fat (50%E ↑ FAT) ± Dex. There was a significant interaction between energy level and Dex such that the increase in body catabolism due to 50%E from ↑ CHO or ↑ FAT was reduced by ∼50%, although the amount of body weight and nitrogen lost over 7 d was significantly greater with 50%E than with AE. AE+Dex induced a 60% increase in liver mass, whereas 50%E+Dex reduced the increase to 26%. AE+Dex induced a 5-fold increase in serum insulin level, whereas 50%E+Dex normalized the insulin to glucose ratio. Serum IGF-I levels were reduced 14-18% by Dex and 30% by 50%E. Hepatic immunoreactive IGF-I was significantly correlated with serum IGF-I and nitrogen balance. 50%E ↑ CHO and 50%E ↑ FAT had differential effects on hepatic IGF-I mRNA with a 40% decrease in IGF-I mRNA due to 50%E ↑ FAT+Dex. In summary, ↑ CHO or ↑ FAT hypoenergetic PN with adequate protein had similar effects in normalizing hyperinsulinemia, attenuating hepatomegaly, and reducing the increment, but not the total amount of body protein catabolism, induced by glucocorticoid excess.

Original languageEnglish (US)
Pages (from-to)479-485
Number of pages7
JournalJournal of Nutrition
Issue number3
Publication statusPublished - Mar 1 2005



  • Glucocorticoids
  • Insulin
  • Insulin-like growth factor-I
  • Nitrogen catabolism

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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