Hygromycin B resistance mediates elimination of Leishmania virus from persistently infected parasites

Young Tae Ro, Scott M. Scheffter, Jean L. Patterson

    Research output: Contribution to journalArticlepeer-review

    11 Scopus citations

    Abstract

    A series of pX63-HYG derivatives encoding Leishmania RNA virus 1-4 (LRV1-4) sequences were electroporated into cells of Leishmania strain M4147, a virus-infected strain of L. guyanensis. After 6 weeks of drug selection (hygromycin B), transfected parasites lacked detectable quantifies of viral genomic double-stranded RNA, viral capsid protein, and RNA-dependent RNA polymerase (RDRP) activity. Evidence of viral infection was not recovered upon removal of the drug. While viral RNA transcripts were produced from electroporated expression vectors, as determined by reverse transcription- PCR, viral antigens were not detected, suggesting that the antiviral effects of hygromycin B are mediated through translation inhibition. A short-term selection study suggests that the LRV1-4 elimination may not only be a function of hygromycin B as a protein synthesis inhibitor but also possibly related to the mechanism of hygromycin B resistance in Leishmania strains.

    Original languageEnglish (US)
    Pages (from-to)8991-8998
    Number of pages8
    JournalJournal of virology
    Volume71
    Issue number12
    DOIs
    StatePublished - Dec 1997

    ASJC Scopus subject areas

    • Microbiology
    • Immunology
    • Insect Science
    • Virology

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