Hydrogen sulfide ameliorates aging-associated changes in the kidney

Hak Joo Lee, Denis Feliers, Jeffrey L. Barnes, Sae Oh, Goutam Ghosh-choudhury, Vivian Diaz, Veronica Galvan Hart, John R Strong, James F Nelson, Adam Salmon, Christopher G. Kevil, Balakuntalam S Kasinath

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Aging is associated with replacement of normal kidney parenchyma by fibrosis. Because hydrogen sulfide (H2S) ameliorates kidney fibrosis in disease models, we examined its status in the aging kidney. In the first study, we examined kidney cortical H2S metabolism and signaling pathways related to synthesis of proteins including matrix proteins in young and old male C57BL/6 mice. In old mice, increase in renal cortical content of matrix protein involved in fibrosis was associated with decreased H2S generation and AMPK activity, and activation of insulin receptor (IR)/IRS-2-Akt-mTORC1-mRNA translation signaling axis that can lead to increase in protein synthesis. In the second study, we randomized 18–19 month-old male C57BL/6 mice to receive 30 μmol/L sodium hydrosulfide (NaHS) in drinking water vs. water alone (control) for 5 months. Administration of NaHS increased plasma free sulfide levels. NaHS inhibited the increase in kidney cortical content of matrix proteins involved in fibrosis and ameliorated glomerulosclerosis. NaHS restored AMPK activity and inhibited activation of IR/IRS-2-Akt-mTORC1-mRNA translation axis. NaHS inhibited age-related increase in kidney cortical content of p21, IL-1β, and IL-6, components of the senescence-associated secretory phenotype. NaHS abolished increase in urinary albumin excretion seen in control mice and reduced serum cystatin C levels suggesting improved glomerular clearance function. We conclude that aging-induced changes in the kidney are associated with H2S deficiency. Administration of H2S ameliorates aging-induced kidney changes probably by inhibiting signaling pathways leading to matrix protein synthesis.

Original languageEnglish (US)
Pages (from-to)1-14
Number of pages14
JournalGeroScience
DOIs
StateAccepted/In press - May 1 2018

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Hydrogen Sulfide
Kidney
Fibrosis
Proteins
AMP-Activated Protein Kinases
Insulin Receptor
Protein Biosynthesis
Inbred C57BL Mouse
Cystatin C
Sulfides
sodium bisulfide
Interleukin-1
Drinking Water
Albumins
Interleukin-6
Phenotype
Water

Keywords

  • AMP-activated protein kinase
  • Insulin receptor signaling
  • Mechanistic target of rapamycin
  • Protein chemistry

ASJC Scopus subject areas

  • Aging
  • Geriatrics and Gerontology

Cite this

Lee, H. J., Feliers, D., Barnes, J. L., Oh, S., Ghosh-choudhury, G., Diaz, V., ... Kasinath, B. S. (Accepted/In press). Hydrogen sulfide ameliorates aging-associated changes in the kidney. GeroScience, 1-14. https://doi.org/10.1007/s11357-018-0018-y

Hydrogen sulfide ameliorates aging-associated changes in the kidney. / Lee, Hak Joo; Feliers, Denis; Barnes, Jeffrey L.; Oh, Sae; Ghosh-choudhury, Goutam; Diaz, Vivian; Galvan Hart, Veronica; Strong, John R; Nelson, James F; Salmon, Adam; Kevil, Christopher G.; Kasinath, Balakuntalam S.

In: GeroScience, 01.05.2018, p. 1-14.

Research output: Contribution to journalArticle

Lee, Hak Joo ; Feliers, Denis ; Barnes, Jeffrey L. ; Oh, Sae ; Ghosh-choudhury, Goutam ; Diaz, Vivian ; Galvan Hart, Veronica ; Strong, John R ; Nelson, James F ; Salmon, Adam ; Kevil, Christopher G. ; Kasinath, Balakuntalam S. / Hydrogen sulfide ameliorates aging-associated changes in the kidney. In: GeroScience. 2018 ; pp. 1-14.
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