Hydrogen peroxide production in cultured RPE cells exposed to near infrared lasers

Michael L. Denton, Debbie M. Eikum, David J. Stolarski, Gary D. Noojin, Robert J. Thomas, Randolph D. Glickman, Benjamin A. Rockwell

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

Hydrogen peroxide (H 2O 2) in situ synthesis within cultures of retinal pigment epithelial cells exposed to near-IR (NIR) continuous wave (CW) and mode-locked lasers is investigated. Any production of H 2O 2 as a consequence of NIR laser exposure is indicative of a multiphoton absorption event, resulting in an intracellular photochemical reaction. Comparisons of mode-locked and CW laser threshold energies required for the photo-oxidative reaction provide support for an enhancement in multiphoton absorption in tissues exposed to the high peak powers inherent in mode-locked laser beams. Due to different fluorescent staining properties our results indicate different mechanisms for oxidative stress from CW and mode-locked laser exposure.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsS.L. Jacques, D.D. Duncan, S.J. Kirkpatrick, A. Kriete
Pages150-155
Number of pages6
Volume4617
DOIs
StatePublished - 2002
EventLaser Tissue Interaction XIII: Photochemical, Photothermal and Photomechanical - San Jose, CA, United States
Duration: Jan 20 2002Jan 23 2002

Other

OtherLaser Tissue Interaction XIII: Photochemical, Photothermal and Photomechanical
CountryUnited States
CitySan Jose, CA
Period1/20/021/23/02

Keywords

  • Fluorescence microscopy
  • Mode-locked
  • Multi-photon
  • RPE cells

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

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    Denton, M. L., Eikum, D. M., Stolarski, D. J., Noojin, G. D., Thomas, R. J., Glickman, R. D., & Rockwell, B. A. (2002). Hydrogen peroxide production in cultured RPE cells exposed to near infrared lasers. In S. L. Jacques, D. D. Duncan, S. J. Kirkpatrick, & A. Kriete (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 4617, pp. 150-155) https://doi.org/10.1117/12.472518