Abstract
Hydrogen peroxide (H 2O 2) in situ synthesis within cultures of retinal pigment epithelial cells exposed to near-IR (NIR) continuous wave (CW) and mode-locked lasers is investigated. Any production of H 2O 2 as a consequence of NIR laser exposure is indicative of a multiphoton absorption event, resulting in an intracellular photochemical reaction. Comparisons of mode-locked and CW laser threshold energies required for the photo-oxidative reaction provide support for an enhancement in multiphoton absorption in tissues exposed to the high peak powers inherent in mode-locked laser beams. Due to different fluorescent staining properties our results indicate different mechanisms for oxidative stress from CW and mode-locked laser exposure.
Original language | English (US) |
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Title of host publication | Proceedings of SPIE - The International Society for Optical Engineering |
Editors | S.L. Jacques, D.D. Duncan, S.J. Kirkpatrick, A. Kriete |
Pages | 150-155 |
Number of pages | 6 |
Volume | 4617 |
DOIs | |
State | Published - 2002 |
Event | Laser Tissue Interaction XIII: Photochemical, Photothermal and Photomechanical - San Jose, CA, United States Duration: Jan 20 2002 → Jan 23 2002 |
Other
Other | Laser Tissue Interaction XIII: Photochemical, Photothermal and Photomechanical |
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Country/Territory | United States |
City | San Jose, CA |
Period | 1/20/02 → 1/23/02 |
Keywords
- Fluorescence microscopy
- Mode-locked
- Multi-photon
- RPE cells
ASJC Scopus subject areas
- Electrical and Electronic Engineering
- Condensed Matter Physics