Hydrogen peroxide production in cultured RPE cells exposed to near infrared lasers

Michael L. Denton; Debbie M. Eikum; David J. Stolarski; Gary D. Noojin; Robert J. Thomas; Randolph D. Glickman; Benjamin A. Rockwell

doi:10.1117/12.472518

Hydrogen peroxide production in cultured RPE cells exposed to near infrared lasers

Michael L. Denton, Debbie M. Eikum, David J. Stolarski, Gary D. Noojin, Robert J. Thomas, Randolph D. Glickman, Benjamin A. Rockwell

Ophthalmology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

2 Scopus citations

Abstract

Hydrogen peroxide (H ₂O ₂) in situ synthesis within cultures of retinal pigment epithelial cells exposed to near-IR (NIR) continuous wave (CW) and mode-locked lasers is investigated. Any production of H ₂O ₂ as a consequence of NIR laser exposure is indicative of a multiphoton absorption event, resulting in an intracellular photochemical reaction. Comparisons of mode-locked and CW laser threshold energies required for the photo-oxidative reaction provide support for an enhancement in multiphoton absorption in tissues exposed to the high peak powers inherent in mode-locked laser beams. Due to different fluorescent staining properties our results indicate different mechanisms for oxidative stress from CW and mode-locked laser exposure.

Original language	English (US)
Title of host publication	Proceedings of SPIE - The International Society for Optical Engineering
Editors	S.L. Jacques, D.D. Duncan, S.J. Kirkpatrick, A. Kriete
Pages	150-155
Number of pages	6
Volume	4617
DOIs	https://doi.org/10.1117/12.472518
State	Published - 2002
Event	Laser Tissue Interaction XIII: Photochemical, Photothermal and Photomechanical - San Jose, CA, United States Duration: Jan 20 2002 → Jan 23 2002

Other

Other	Laser Tissue Interaction XIII: Photochemical, Photothermal and Photomechanical
Country	United States
City	San Jose, CA
Period	1/20/02 → 1/23/02

Keywords

Fluorescence microscopy
Mode-locked
Multi-photon
RPE cells

ASJC Scopus subject areas

Electrical and Electronic Engineering
Condensed Matter Physics

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10.1117/12.472518

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Denton, M. L., Eikum, D. M., Stolarski, D. J., Noojin, G. D., Thomas, R. J., Glickman, R. D., & Rockwell, B. A. (2002). Hydrogen peroxide production in cultured RPE cells exposed to near infrared lasers. In S. L. Jacques, D. D. Duncan, S. J. Kirkpatrick, & A. Kriete (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 4617, pp. 150-155) https://doi.org/10.1117/12.472518

Hydrogen peroxide production in cultured RPE cells exposed to near infrared lasers. / Denton, Michael L.; Eikum, Debbie M.; Stolarski, David J.; Noojin, Gary D.; Thomas, Robert J.; Glickman, Randolph D.; Rockwell, Benjamin A.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / S.L. Jacques; D.D. Duncan; S.J. Kirkpatrick; A. Kriete. Vol. 4617 2002. p. 150-155.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Denton, ML, Eikum, DM, Stolarski, DJ, Noojin, GD, Thomas, RJ, Glickman, RD & Rockwell, BA 2002, Hydrogen peroxide production in cultured RPE cells exposed to near infrared lasers. in SL Jacques, DD Duncan, SJ Kirkpatrick & A Kriete (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 4617, pp. 150-155, Laser Tissue Interaction XIII: Photochemical, Photothermal and Photomechanical, San Jose, CA, United States, 1/20/02. https://doi.org/10.1117/12.472518

Denton, Michael L. ; Eikum, Debbie M. ; Stolarski, David J. ; Noojin, Gary D. ; Thomas, Robert J. ; Glickman, Randolph D. ; Rockwell, Benjamin A. / Hydrogen peroxide production in cultured RPE cells exposed to near infrared lasers. Proceedings of SPIE - The International Society for Optical Engineering. editor / S.L. Jacques ; D.D. Duncan ; S.J. Kirkpatrick ; A. Kriete. Vol. 4617 2002. pp. 150-155

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title = "Hydrogen peroxide production in cultured RPE cells exposed to near infrared lasers",

abstract = "Hydrogen peroxide (H 2O 2) in situ synthesis within cultures of retinal pigment epithelial cells exposed to near-IR (NIR) continuous wave (CW) and mode-locked lasers is investigated. Any production of H 2O 2 as a consequence of NIR laser exposure is indicative of a multiphoton absorption event, resulting in an intracellular photochemical reaction. Comparisons of mode-locked and CW laser threshold energies required for the photo-oxidative reaction provide support for an enhancement in multiphoton absorption in tissues exposed to the high peak powers inherent in mode-locked laser beams. Due to different fluorescent staining properties our results indicate different mechanisms for oxidative stress from CW and mode-locked laser exposure. ",

keywords = "Fluorescence microscopy, Mode-locked, Multi-photon, RPE cells",

author = "Denton, {Michael L.} and Eikum, {Debbie M.} and Stolarski, {David J.} and Noojin, {Gary D.} and Thomas, {Robert J.} and Glickman, {Randolph D.} and Rockwell, {Benjamin A.}",

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AU - Denton, Michael L.

AU - Eikum, Debbie M.

AU - Stolarski, David J.

AU - Noojin, Gary D.

AU - Thomas, Robert J.

AU - Glickman, Randolph D.

AU - Rockwell, Benjamin A.

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N2 - Hydrogen peroxide (H 2O 2) in situ synthesis within cultures of retinal pigment epithelial cells exposed to near-IR (NIR) continuous wave (CW) and mode-locked lasers is investigated. Any production of H 2O 2 as a consequence of NIR laser exposure is indicative of a multiphoton absorption event, resulting in an intracellular photochemical reaction. Comparisons of mode-locked and CW laser threshold energies required for the photo-oxidative reaction provide support for an enhancement in multiphoton absorption in tissues exposed to the high peak powers inherent in mode-locked laser beams. Due to different fluorescent staining properties our results indicate different mechanisms for oxidative stress from CW and mode-locked laser exposure.

AB - Hydrogen peroxide (H 2O 2) in situ synthesis within cultures of retinal pigment epithelial cells exposed to near-IR (NIR) continuous wave (CW) and mode-locked lasers is investigated. Any production of H 2O 2 as a consequence of NIR laser exposure is indicative of a multiphoton absorption event, resulting in an intracellular photochemical reaction. Comparisons of mode-locked and CW laser threshold energies required for the photo-oxidative reaction provide support for an enhancement in multiphoton absorption in tissues exposed to the high peak powers inherent in mode-locked laser beams. Due to different fluorescent staining properties our results indicate different mechanisms for oxidative stress from CW and mode-locked laser exposure.

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KW - Multi-photon

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