Humoral and cell-mediated immune responses in fully allogeneic bone marrow chimera in mice

K. Onoe, G. Fernandes, R. A. Good

Research output: Contribution to journalArticle

123 Citations (Scopus)

Abstract

AKR mice were protected from lethal irradiation and established as long-lived chimeras by transplanting allogeneic C57BL/6 (B6) bone marrow that had been treated in vitro with anti-Thy-1 antiserum without complement. In these chimeras, which were designated [B6 → AKR], virtually all the thymus and spleen cells were shown to be derived from the B6 donor; several immune functions studied in these chimeras were as follows: (a) The chimeric mice were tolerant of histocompatibility antigens of both donor and recipient strain and nearly fully reactive to antigens of third party, as revealed by Simonsen's splenomegaly assay. The tolerance of these chimeras could not be attributed to suppressor cells but was compatible with clonal depletion. (b) Proliferative responses to concanavalin A, phytohemagglutinin, and lipopolysaccharide as well as natural killer and antibody-dependent cell-mediated cytotoxicity activity of the chimeric mice was normal. (c) Plaque-forming cell (PFC) assays of antibody response to sheep erythrocytes (SRBC) showed gross deficiency in the primary response of the [B6 → AKR] and [AKR → B6] chimeras. By contrast, [B6-H-2(k)(E(k)) → AKR] H-2-compatible chimeras and [AKR → AKR] syngeneic marrow transplanted mice had normal primary PFC responses. PFC responses after secondary stimulation with SRBC, however, revealed vigorous direct plaque formation and substantial but somewhat smaller indirect plaque formation in the [B6 → AKR] chimeras. This observation favors operationally the concept of adaptive differentiation proposed by Katz et al. (d) Analysis of ability of the chimeras to develop and express delayed-type hypersensitivity responses to contact sensitizer (2,4-dinitro-1-fluorobenzene [DNFB]) showed no apparent immunodeficiency of either chimeras to this form of immunization. Development of immunologic tolerance to DNFB, however, was grossly deficient in [B6 → AKR] chimeras but normal in [AKR → AKR], [B6 → B6], and [E(k) → AKR] chimeras. These findings indicate that full chimeras across major histocompatibility complex have considerable immunologic vigor even though primary immune responses that require histocompatibility between interacting cell types are initially defective.

Original languageEnglish (US)
Pages (from-to)115-132
Number of pages18
JournalJournal of Experimental Medicine
Volume151
Issue number1
StatePublished - 1980
Externally publishedYes

Fingerprint

Bone Marrow
Fluorobenzenes
Inbred AKR Mouse
Antibody-Dependent Cell Cytotoxicity
Histocompatibility Antigens
Histocompatibility
Splenomegaly
Delayed Hypersensitivity
Phytohemagglutinins
Concanavalin A
Major Histocompatibility Complex
Thymus Gland
Antibody Formation
Lipopolysaccharides
Immune Sera
Immunization
Sheep
Spleen
Erythrocytes
Antigens

ASJC Scopus subject areas

  • Immunology

Cite this

Humoral and cell-mediated immune responses in fully allogeneic bone marrow chimera in mice. / Onoe, K.; Fernandes, G.; Good, R. A.

In: Journal of Experimental Medicine, Vol. 151, No. 1, 1980, p. 115-132.

Research output: Contribution to journalArticle

Onoe, K. ; Fernandes, G. ; Good, R. A. / Humoral and cell-mediated immune responses in fully allogeneic bone marrow chimera in mice. In: Journal of Experimental Medicine. 1980 ; Vol. 151, No. 1. pp. 115-132.
@article{5509732625d347aca3e99704a56f279d,
title = "Humoral and cell-mediated immune responses in fully allogeneic bone marrow chimera in mice",
abstract = "AKR mice were protected from lethal irradiation and established as long-lived chimeras by transplanting allogeneic C57BL/6 (B6) bone marrow that had been treated in vitro with anti-Thy-1 antiserum without complement. In these chimeras, which were designated [B6 → AKR], virtually all the thymus and spleen cells were shown to be derived from the B6 donor; several immune functions studied in these chimeras were as follows: (a) The chimeric mice were tolerant of histocompatibility antigens of both donor and recipient strain and nearly fully reactive to antigens of third party, as revealed by Simonsen's splenomegaly assay. The tolerance of these chimeras could not be attributed to suppressor cells but was compatible with clonal depletion. (b) Proliferative responses to concanavalin A, phytohemagglutinin, and lipopolysaccharide as well as natural killer and antibody-dependent cell-mediated cytotoxicity activity of the chimeric mice was normal. (c) Plaque-forming cell (PFC) assays of antibody response to sheep erythrocytes (SRBC) showed gross deficiency in the primary response of the [B6 → AKR] and [AKR → B6] chimeras. By contrast, [B6-H-2(k)(E(k)) → AKR] H-2-compatible chimeras and [AKR → AKR] syngeneic marrow transplanted mice had normal primary PFC responses. PFC responses after secondary stimulation with SRBC, however, revealed vigorous direct plaque formation and substantial but somewhat smaller indirect plaque formation in the [B6 → AKR] chimeras. This observation favors operationally the concept of adaptive differentiation proposed by Katz et al. (d) Analysis of ability of the chimeras to develop and express delayed-type hypersensitivity responses to contact sensitizer (2,4-dinitro-1-fluorobenzene [DNFB]) showed no apparent immunodeficiency of either chimeras to this form of immunization. Development of immunologic tolerance to DNFB, however, was grossly deficient in [B6 → AKR] chimeras but normal in [AKR → AKR], [B6 → B6], and [E(k) → AKR] chimeras. These findings indicate that full chimeras across major histocompatibility complex have considerable immunologic vigor even though primary immune responses that require histocompatibility between interacting cell types are initially defective.",
author = "K. Onoe and G. Fernandes and Good, {R. A.}",
year = "1980",
language = "English (US)",
volume = "151",
pages = "115--132",
journal = "Journal of Experimental Medicine",
issn = "0022-1007",
publisher = "Rockefeller University Press",
number = "1",

}

TY - JOUR

T1 - Humoral and cell-mediated immune responses in fully allogeneic bone marrow chimera in mice

AU - Onoe, K.

AU - Fernandes, G.

AU - Good, R. A.

PY - 1980

Y1 - 1980

N2 - AKR mice were protected from lethal irradiation and established as long-lived chimeras by transplanting allogeneic C57BL/6 (B6) bone marrow that had been treated in vitro with anti-Thy-1 antiserum without complement. In these chimeras, which were designated [B6 → AKR], virtually all the thymus and spleen cells were shown to be derived from the B6 donor; several immune functions studied in these chimeras were as follows: (a) The chimeric mice were tolerant of histocompatibility antigens of both donor and recipient strain and nearly fully reactive to antigens of third party, as revealed by Simonsen's splenomegaly assay. The tolerance of these chimeras could not be attributed to suppressor cells but was compatible with clonal depletion. (b) Proliferative responses to concanavalin A, phytohemagglutinin, and lipopolysaccharide as well as natural killer and antibody-dependent cell-mediated cytotoxicity activity of the chimeric mice was normal. (c) Plaque-forming cell (PFC) assays of antibody response to sheep erythrocytes (SRBC) showed gross deficiency in the primary response of the [B6 → AKR] and [AKR → B6] chimeras. By contrast, [B6-H-2(k)(E(k)) → AKR] H-2-compatible chimeras and [AKR → AKR] syngeneic marrow transplanted mice had normal primary PFC responses. PFC responses after secondary stimulation with SRBC, however, revealed vigorous direct plaque formation and substantial but somewhat smaller indirect plaque formation in the [B6 → AKR] chimeras. This observation favors operationally the concept of adaptive differentiation proposed by Katz et al. (d) Analysis of ability of the chimeras to develop and express delayed-type hypersensitivity responses to contact sensitizer (2,4-dinitro-1-fluorobenzene [DNFB]) showed no apparent immunodeficiency of either chimeras to this form of immunization. Development of immunologic tolerance to DNFB, however, was grossly deficient in [B6 → AKR] chimeras but normal in [AKR → AKR], [B6 → B6], and [E(k) → AKR] chimeras. These findings indicate that full chimeras across major histocompatibility complex have considerable immunologic vigor even though primary immune responses that require histocompatibility between interacting cell types are initially defective.

AB - AKR mice were protected from lethal irradiation and established as long-lived chimeras by transplanting allogeneic C57BL/6 (B6) bone marrow that had been treated in vitro with anti-Thy-1 antiserum without complement. In these chimeras, which were designated [B6 → AKR], virtually all the thymus and spleen cells were shown to be derived from the B6 donor; several immune functions studied in these chimeras were as follows: (a) The chimeric mice were tolerant of histocompatibility antigens of both donor and recipient strain and nearly fully reactive to antigens of third party, as revealed by Simonsen's splenomegaly assay. The tolerance of these chimeras could not be attributed to suppressor cells but was compatible with clonal depletion. (b) Proliferative responses to concanavalin A, phytohemagglutinin, and lipopolysaccharide as well as natural killer and antibody-dependent cell-mediated cytotoxicity activity of the chimeric mice was normal. (c) Plaque-forming cell (PFC) assays of antibody response to sheep erythrocytes (SRBC) showed gross deficiency in the primary response of the [B6 → AKR] and [AKR → B6] chimeras. By contrast, [B6-H-2(k)(E(k)) → AKR] H-2-compatible chimeras and [AKR → AKR] syngeneic marrow transplanted mice had normal primary PFC responses. PFC responses after secondary stimulation with SRBC, however, revealed vigorous direct plaque formation and substantial but somewhat smaller indirect plaque formation in the [B6 → AKR] chimeras. This observation favors operationally the concept of adaptive differentiation proposed by Katz et al. (d) Analysis of ability of the chimeras to develop and express delayed-type hypersensitivity responses to contact sensitizer (2,4-dinitro-1-fluorobenzene [DNFB]) showed no apparent immunodeficiency of either chimeras to this form of immunization. Development of immunologic tolerance to DNFB, however, was grossly deficient in [B6 → AKR] chimeras but normal in [AKR → AKR], [B6 → B6], and [E(k) → AKR] chimeras. These findings indicate that full chimeras across major histocompatibility complex have considerable immunologic vigor even though primary immune responses that require histocompatibility between interacting cell types are initially defective.

UR - http://www.scopus.com/inward/record.url?scp=0018851278&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018851278&partnerID=8YFLogxK

M3 - Article

C2 - 6985646

AN - SCOPUS:0018851278

VL - 151

SP - 115

EP - 132

JO - Journal of Experimental Medicine

JF - Journal of Experimental Medicine

SN - 0022-1007

IS - 1

ER -