Human transferrin: cDNA characterization and chromosomal localization

F. Yang, J. B. Lum, J. R. McGill, C. M. Moore, S. L. Naylor, P. H. van Bragt, W. D. Baldwin, B. H. Bowman

Research output: Contribution to journalArticle

266 Citations (Scopus)

Abstract

Transferrin (Tf) is the major iron binding protein in vertebrate serum. It shares homologous amino acid sequences with four other proteins: lactotransferrin, ovotransferrin, melanoma antigen p97, and HuBlym-1. Antigen p97 and the Tf receptor genes have been mapped on human chromosome 3. The goal of the study described here was to initiate the characterization of the Tf gene by identifying and characterizing its cDNA and mapping its chromosomal location. Recombinant plasmids containing human cDNA encoding Tf have been isolated by screening an adult human liver library with a mixed oligonucleotide probe. Within the 2.3 kilobase pairs of Tf cDNA analyzed, there is a probable leader sequence encoded by 57 nucleotides followed by 2037 nucleotides that encode the homologous amino and carboxyl domains. During evolution, three areas of the homologous amino and carboxyl domains have been strongly conserved, possibly reflecting functional constraints associated with iron binding. Chromosomal mapping by in situ hybridization and somatic cell hybrid analysis indicate that the Tf gene is located at q21-25 on human chromosome 3, consistent with linkage of the Tf, Tf receptor, and melanoma p97 loci.

Original languageEnglish (US)
Pages (from-to)2752-2756
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume81
Issue number9 I
StatePublished - 1984
Externally publishedYes

Fingerprint

Transferrin
Complementary DNA
Transferrin Receptors
Chromosomes, Human, Pair 3
Human Chromosomes
Nucleotides
Conalbumin
Iron-Binding Proteins
Genes
Melanoma-Specific Antigens
Amino Acid Sequence Homology
Lactoferrin
Oligonucleotide Probes
Hybrid Cells
In Situ Hybridization
Vertebrates
Melanoma
Plasmids
Iron
Antigens

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Yang, F., Lum, J. B., McGill, J. R., Moore, C. M., Naylor, S. L., van Bragt, P. H., ... Bowman, B. H. (1984). Human transferrin: cDNA characterization and chromosomal localization. Proceedings of the National Academy of Sciences of the United States of America, 81(9 I), 2752-2756.

Human transferrin : cDNA characterization and chromosomal localization. / Yang, F.; Lum, J. B.; McGill, J. R.; Moore, C. M.; Naylor, S. L.; van Bragt, P. H.; Baldwin, W. D.; Bowman, B. H.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 81, No. 9 I, 1984, p. 2752-2756.

Research output: Contribution to journalArticle

Yang, F, Lum, JB, McGill, JR, Moore, CM, Naylor, SL, van Bragt, PH, Baldwin, WD & Bowman, BH 1984, 'Human transferrin: cDNA characterization and chromosomal localization', Proceedings of the National Academy of Sciences of the United States of America, vol. 81, no. 9 I, pp. 2752-2756.
Yang, F. ; Lum, J. B. ; McGill, J. R. ; Moore, C. M. ; Naylor, S. L. ; van Bragt, P. H. ; Baldwin, W. D. ; Bowman, B. H. / Human transferrin : cDNA characterization and chromosomal localization. In: Proceedings of the National Academy of Sciences of the United States of America. 1984 ; Vol. 81, No. 9 I. pp. 2752-2756.
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