Human Pso4 is a metnase (SETMAR)-binding partner that regulates metnase function in DNA repair

Brian D. Beck, Su Jung Park, Young Ju Lee, Yaritzabel Roman, Robert A. Hromas, Suk Hee Lee

Research output: Contribution to journalArticlepeer-review

57 Scopus citations

Abstract

Metnase, also known as SETMAR, is a SET and transposase fusion protein with an undefined role in mammalian DNA repair. The SET domain is responsible for histone lysine methyltransferase activity at histone 3K4and K36, whereas the transposase domain possesses 5′-terminal inverted repeat (TIR)-specific DNA binding, DNA looping, and DNA cleavage activities. Although the transposase domain is essential for Metnase function in DNA repair, it is not clear how a protein with sequence-specific DNA binding activity plays a role in DNA repair. Here, we show that human homolog of the ScPSO4/PRP19 (hPso4) forms a stable complex with Metnase on both TIR and non-TIR DNA. The transposase domain essential for Metnase-TIR interaction is not sufficient for its interaction with non-TIRDNA in the presence of hPso4. In vivo, hPso4 is induced and co-localized with Metnase following ionizing radiation treatment. Cells treated with hPso4-siRNA failed to show Metnase localization at DSB sites and Metnase-mediated stimulation of DNA end joining coupled to genomic integration, suggesting that hPso4 is necessary to bring Metnase to the DSB sites for its function(s) in DNA repair.

Original languageEnglish (US)
Pages (from-to)9023-9030
Number of pages8
JournalJournal of Biological Chemistry
Volume283
Issue number14
DOIs
StatePublished - Apr 4 2008

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Human Pso4 is a metnase (SETMAR)-binding partner that regulates metnase function in DNA repair'. Together they form a unique fingerprint.

Cite this