Human inositol 1,4,5-trisphosphate type-1 receptor, InsP3R1

Structure, function, regulation of expression and chromosomal localization

N. Yamada, Y. Makino, Robert A Clark, D. W. Pearson, M. G. Mattei, J. L. Guenet, E. Ohama, I. Fujino, A. Miyawaki, T. Furuichi, K. Mikoshiba

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

We have isolated cDNA clones encoding an inositol 1,4,5-trisphosphate receptor type 1 (InsP3R1) from human uteri and a leukaemic cell line, HL-60. Northern-blot analysis showed that approx. 10 kb of InsP3R1 mRNA is expressed in human uteri, oviducts and HL-60 cells. The predicted amino acid sequence of human InsP3R1 (2695 amino acids) has 99% identity with that of the mouse SI-/SII- splicing counterpart. Western-blot analysis with anti-(mouse InsP3R1) antibodies showed that InsP3R1 protein of human uteri and oviducts of approx 220 kDa is immunostained. Northern-blot analysis of HL-60 cell differentiation along the neutrophilic lineage induced by retinoic acid or dimethylsulphoxide showed an accompanying enhanced expression of InsP3R1 mRNA. Immunohistochemical analysis of the cerebella of spinocerebellar degeneration patients showed a variable loss of Purkinje cells with an altered pattern of immunostaining. The InsP3R1 gene (Insp3r1) was localized to the 3P25-26 region of human chromosome 3. The data presented here clearly show that InsP3R1 exists widely in human tissues and may play critical roles in various kinds of cellular functions.

Original languageEnglish (US)
Pages (from-to)781-790
Number of pages10
JournalBiochemical Journal
Volume302
Issue number3
StatePublished - 1994
Externally publishedYes

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Inositol 1,4,5-Trisphosphate Receptors
Amino Acids
Messenger RNA
Uterus
Chromosomes
Dimethyl Sulfoxide
Tretinoin
Oviducts
HL-60 Cells
Complementary DNA
Genes
Northern Blotting
Cells
Tissue
Antibodies
Spinocerebellar Degenerations
Chromosomes, Human, Pair 3
Purkinje Cells
Human Chromosomes
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Yamada, N., Makino, Y., Clark, R. A., Pearson, D. W., Mattei, M. G., Guenet, J. L., ... Mikoshiba, K. (1994). Human inositol 1,4,5-trisphosphate type-1 receptor, InsP3R1: Structure, function, regulation of expression and chromosomal localization. Biochemical Journal, 302(3), 781-790.

Human inositol 1,4,5-trisphosphate type-1 receptor, InsP3R1 : Structure, function, regulation of expression and chromosomal localization. / Yamada, N.; Makino, Y.; Clark, Robert A; Pearson, D. W.; Mattei, M. G.; Guenet, J. L.; Ohama, E.; Fujino, I.; Miyawaki, A.; Furuichi, T.; Mikoshiba, K.

In: Biochemical Journal, Vol. 302, No. 3, 1994, p. 781-790.

Research output: Contribution to journalArticle

Yamada, N, Makino, Y, Clark, RA, Pearson, DW, Mattei, MG, Guenet, JL, Ohama, E, Fujino, I, Miyawaki, A, Furuichi, T & Mikoshiba, K 1994, 'Human inositol 1,4,5-trisphosphate type-1 receptor, InsP3R1: Structure, function, regulation of expression and chromosomal localization', Biochemical Journal, vol. 302, no. 3, pp. 781-790.
Yamada, N. ; Makino, Y. ; Clark, Robert A ; Pearson, D. W. ; Mattei, M. G. ; Guenet, J. L. ; Ohama, E. ; Fujino, I. ; Miyawaki, A. ; Furuichi, T. ; Mikoshiba, K. / Human inositol 1,4,5-trisphosphate type-1 receptor, InsP3R1 : Structure, function, regulation of expression and chromosomal localization. In: Biochemical Journal. 1994 ; Vol. 302, No. 3. pp. 781-790.
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abstract = "We have isolated cDNA clones encoding an inositol 1,4,5-trisphosphate receptor type 1 (InsP3R1) from human uteri and a leukaemic cell line, HL-60. Northern-blot analysis showed that approx. 10 kb of InsP3R1 mRNA is expressed in human uteri, oviducts and HL-60 cells. The predicted amino acid sequence of human InsP3R1 (2695 amino acids) has 99{\%} identity with that of the mouse SI-/SII- splicing counterpart. Western-blot analysis with anti-(mouse InsP3R1) antibodies showed that InsP3R1 protein of human uteri and oviducts of approx 220 kDa is immunostained. Northern-blot analysis of HL-60 cell differentiation along the neutrophilic lineage induced by retinoic acid or dimethylsulphoxide showed an accompanying enhanced expression of InsP3R1 mRNA. Immunohistochemical analysis of the cerebella of spinocerebellar degeneration patients showed a variable loss of Purkinje cells with an altered pattern of immunostaining. The InsP3R1 gene (Insp3r1) was localized to the 3P25-26 region of human chromosome 3. The data presented here clearly show that InsP3R1 exists widely in human tissues and may play critical roles in various kinds of cellular functions.",
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