TY - JOUR
T1 - Human immunodeficiency virus type 1 causes productive infection of macrophages in primary placental cell cultures
AU - Mc Gann, Kathleen A.
AU - Collman, Ronald
AU - Kolson, Dennis L.
AU - Gonzalez-Scarano, Francisco
AU - Coukos, George
AU - Coutifaris, Christos
AU - Strauss, Jerome F.
AU - Nathanson, Neal
PY - 1994/4
Y1 - 1994/4
N2 - To characterize the role of the placenta in vertical transmission of human immunodeficiency virus type 1 (HIV-1), the susceptibility of primary human placental cultures and of transformed trophoblast cell lines to infection by several HIV-1 isolates was examined. Placental cultures supported the replication of all strains tested, including lymphocyte-, macrophage-, and amphotropic isolates. All viruses replicated to modestlevels, with production of both viral antigen and infectious virus in the culture supernatants. Placental cells demonstrated a pattern of permissiveness for HIV-1 isolates distinct from that seen with lymphocytes, blood-derived macrophages, or T cell lines. Immunofluorescent staining showed that 5%-10% of the cultured placental cells expressed viral antigens, and double labeling revealed that the HIV-positive cells were macrophages not trophoblasts. None of the trophoblast cell lines (JEG-3, Jar, BeWo, HP-W1) could be infected by HIV. These results support the hypothesis that infection ofthe placenta could playa role in maternofetal transmission of HIV-1 and suggest that the placental macrophage is likely to be the primary cell type responsible.
AB - To characterize the role of the placenta in vertical transmission of human immunodeficiency virus type 1 (HIV-1), the susceptibility of primary human placental cultures and of transformed trophoblast cell lines to infection by several HIV-1 isolates was examined. Placental cultures supported the replication of all strains tested, including lymphocyte-, macrophage-, and amphotropic isolates. All viruses replicated to modestlevels, with production of both viral antigen and infectious virus in the culture supernatants. Placental cells demonstrated a pattern of permissiveness for HIV-1 isolates distinct from that seen with lymphocytes, blood-derived macrophages, or T cell lines. Immunofluorescent staining showed that 5%-10% of the cultured placental cells expressed viral antigens, and double labeling revealed that the HIV-positive cells were macrophages not trophoblasts. None of the trophoblast cell lines (JEG-3, Jar, BeWo, HP-W1) could be infected by HIV. These results support the hypothesis that infection ofthe placenta could playa role in maternofetal transmission of HIV-1 and suggest that the placental macrophage is likely to be the primary cell type responsible.
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U2 - 10.1093/infdis/169.4.746
DO - 10.1093/infdis/169.4.746
M3 - Article
C2 - 8133087
AN - SCOPUS:0028343936
VL - 169
SP - 746
EP - 753
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
SN - 0022-1899
IS - 4
ER -