Human immune interferon gene is located on chromosome 12

S. L. Naylor, Alan Y Sakaguchi, T. B. Shows, M. L. Law, D. V. Goeddel, P. W. Gray

Research output: Contribution to journalArticle

129 Citations (Scopus)

Abstract

A cDNA clone for human immune interferon (IFN-γ) gene sequences, plasmid p69, was used to chromosomally map the IFN-γ gene by detecting human IFN-γ gene sequences in DNA isolated from human-rodent somatic cell hybrids. We were able to map the IFN-γ gene by correlating the human chromosomes present in these hybrids with the human specific 8.8 and 2.0 kilobase pair fragments produced by EcoRI digestion of genomic DNA. Southern blot analysis of 37 hybrid cell lines indicated that the gene for IFN-γ was on human chromosome 12. A hybrid containing a portion of chromosome 12 localized the IFN-γ gene to the p1205→qter region.

Original languageEnglish (US)
Pages (from-to)1020-1027
Number of pages8
JournalJournal of Experimental Medicine
Volume157
Issue number3
StatePublished - 1983
Externally publishedYes

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Chromosomes, Human, Pair 12
Interferon-gamma
Interferons
Genes
Hybrid Cells
Human Chromosomes
Southern Blotting
Digestion
Rodentia
Plasmids
Complementary DNA
Clone Cells
Cell Line
DNA

ASJC Scopus subject areas

  • Immunology

Cite this

Naylor, S. L., Sakaguchi, A. Y., Shows, T. B., Law, M. L., Goeddel, D. V., & Gray, P. W. (1983). Human immune interferon gene is located on chromosome 12. Journal of Experimental Medicine, 157(3), 1020-1027.

Human immune interferon gene is located on chromosome 12. / Naylor, S. L.; Sakaguchi, Alan Y; Shows, T. B.; Law, M. L.; Goeddel, D. V.; Gray, P. W.

In: Journal of Experimental Medicine, Vol. 157, No. 3, 1983, p. 1020-1027.

Research output: Contribution to journalArticle

Naylor, SL, Sakaguchi, AY, Shows, TB, Law, ML, Goeddel, DV & Gray, PW 1983, 'Human immune interferon gene is located on chromosome 12', Journal of Experimental Medicine, vol. 157, no. 3, pp. 1020-1027.
Naylor SL, Sakaguchi AY, Shows TB, Law ML, Goeddel DV, Gray PW. Human immune interferon gene is located on chromosome 12. Journal of Experimental Medicine. 1983;157(3):1020-1027.
Naylor, S. L. ; Sakaguchi, Alan Y ; Shows, T. B. ; Law, M. L. ; Goeddel, D. V. ; Gray, P. W. / Human immune interferon gene is located on chromosome 12. In: Journal of Experimental Medicine. 1983 ; Vol. 157, No. 3. pp. 1020-1027.
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