Human herpes virus 8 (KSHV) contamination of peripheral blood and autograft products from multiple myeloma patients

R. A. Vescio, C. H. Wu, L. Zheng, D. Sheen, H. Ma, J. Liu, A. K. Stewart, O. Ballester, S. J. Noga, H. Rugo, C. Freytes, E. Stadtmauer, F. Sahebi, S. Tarantolo, P. Stiff, G. J. Schiller, M. White, C. Jacobs, J. Dipersio, K. C. AndersonJ. R. Berenson

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Human herpesvirus 8 (HHV-8), also known as Kaposi's sarcoma-associated herpesvirus (KSHV), has recently been identified within the bone marrow dendritic cells of multiple myeloma (MM) patients. This virus contains homologues to human cytokines such as IL-6 that could potentially stimulate myeloma cell growth and contribute to disease pathogenesis. Since mobilization chemotherapy may increase circulating dendritic cell numbers, we searched for HHV-8 in peripheral blood mononuclear cells (PBMCs) before and after mobilization chemotherapy given to MM patients. Furthermore, we determined if autograft purging using the CEPRATE SC device would reduce the percentage of HHV-8 infected stem cell products. Only two of the 39 PBMC samples collected prior to mobilization chemotherapy contained PCR detectable virus, yet nine of 37 PBMCs collected on the first day of leukapheresis had detectable HHV-8 (P = 0.016). HHV-8 was more frequently identified in autograft products before vs after Ceprate SC selection (40% vs 15%, P = 0.016). Although the role HHV-8 plays in myeloma pathogenesis remains unclear, these results imply that mobilization chemotherapy increases the numbers of circulating HHV-8-infected dendritic cells within the peripheral blood. In addition, CD34 selection of autograft products in MM patients may reduce the reintroduction of virally infected cells following high-dose chemotherapy.

Original languageEnglish (US)
Pages (from-to)153-160
Number of pages8
JournalBone Marrow Transplantation
Volume25
Issue number2
StatePublished - 2000

Fingerprint

Human Herpesvirus 8
Autografts
Multiple Myeloma
Viruses
Drug Therapy
Dendritic Cells
Blood Cells
Leukapheresis
Bone Marrow Cells
Interleukin-6
Stem Cells
Cell Count
Cytokines
Equipment and Supplies
Polymerase Chain Reaction
Growth

Keywords

  • Autologous transplantation
  • Human herpesvirus 8
  • Kaposi's sarcoma-associated herpesvirus
  • Leukapheresis
  • Multiple myeloma
  • Pathogenesis

ASJC Scopus subject areas

  • Hematology
  • Transplantation

Cite this

Vescio, R. A., Wu, C. H., Zheng, L., Sheen, D., Ma, H., Liu, J., ... Berenson, J. R. (2000). Human herpes virus 8 (KSHV) contamination of peripheral blood and autograft products from multiple myeloma patients. Bone Marrow Transplantation, 25(2), 153-160.

Human herpes virus 8 (KSHV) contamination of peripheral blood and autograft products from multiple myeloma patients. / Vescio, R. A.; Wu, C. H.; Zheng, L.; Sheen, D.; Ma, H.; Liu, J.; Stewart, A. K.; Ballester, O.; Noga, S. J.; Rugo, H.; Freytes, C.; Stadtmauer, E.; Sahebi, F.; Tarantolo, S.; Stiff, P.; Schiller, G. J.; White, M.; Jacobs, C.; Dipersio, J.; Anderson, K. C.; Berenson, J. R.

In: Bone Marrow Transplantation, Vol. 25, No. 2, 2000, p. 153-160.

Research output: Contribution to journalArticle

Vescio, RA, Wu, CH, Zheng, L, Sheen, D, Ma, H, Liu, J, Stewart, AK, Ballester, O, Noga, SJ, Rugo, H, Freytes, C, Stadtmauer, E, Sahebi, F, Tarantolo, S, Stiff, P, Schiller, GJ, White, M, Jacobs, C, Dipersio, J, Anderson, KC & Berenson, JR 2000, 'Human herpes virus 8 (KSHV) contamination of peripheral blood and autograft products from multiple myeloma patients', Bone Marrow Transplantation, vol. 25, no. 2, pp. 153-160.
Vescio, R. A. ; Wu, C. H. ; Zheng, L. ; Sheen, D. ; Ma, H. ; Liu, J. ; Stewart, A. K. ; Ballester, O. ; Noga, S. J. ; Rugo, H. ; Freytes, C. ; Stadtmauer, E. ; Sahebi, F. ; Tarantolo, S. ; Stiff, P. ; Schiller, G. J. ; White, M. ; Jacobs, C. ; Dipersio, J. ; Anderson, K. C. ; Berenson, J. R. / Human herpes virus 8 (KSHV) contamination of peripheral blood and autograft products from multiple myeloma patients. In: Bone Marrow Transplantation. 2000 ; Vol. 25, No. 2. pp. 153-160.
@article{3ba6395973744011b206c0e39231779d,
title = "Human herpes virus 8 (KSHV) contamination of peripheral blood and autograft products from multiple myeloma patients",
abstract = "Human herpesvirus 8 (HHV-8), also known as Kaposi's sarcoma-associated herpesvirus (KSHV), has recently been identified within the bone marrow dendritic cells of multiple myeloma (MM) patients. This virus contains homologues to human cytokines such as IL-6 that could potentially stimulate myeloma cell growth and contribute to disease pathogenesis. Since mobilization chemotherapy may increase circulating dendritic cell numbers, we searched for HHV-8 in peripheral blood mononuclear cells (PBMCs) before and after mobilization chemotherapy given to MM patients. Furthermore, we determined if autograft purging using the CEPRATE SC device would reduce the percentage of HHV-8 infected stem cell products. Only two of the 39 PBMC samples collected prior to mobilization chemotherapy contained PCR detectable virus, yet nine of 37 PBMCs collected on the first day of leukapheresis had detectable HHV-8 (P = 0.016). HHV-8 was more frequently identified in autograft products before vs after Ceprate SC selection (40{\%} vs 15{\%}, P = 0.016). Although the role HHV-8 plays in myeloma pathogenesis remains unclear, these results imply that mobilization chemotherapy increases the numbers of circulating HHV-8-infected dendritic cells within the peripheral blood. In addition, CD34 selection of autograft products in MM patients may reduce the reintroduction of virally infected cells following high-dose chemotherapy.",
keywords = "Autologous transplantation, Human herpesvirus 8, Kaposi's sarcoma-associated herpesvirus, Leukapheresis, Multiple myeloma, Pathogenesis",
author = "Vescio, {R. A.} and Wu, {C. H.} and L. Zheng and D. Sheen and H. Ma and J. Liu and Stewart, {A. K.} and O. Ballester and Noga, {S. J.} and H. Rugo and C. Freytes and E. Stadtmauer and F. Sahebi and S. Tarantolo and P. Stiff and Schiller, {G. J.} and M. White and C. Jacobs and J. Dipersio and Anderson, {K. C.} and Berenson, {J. R.}",
year = "2000",
language = "English (US)",
volume = "25",
pages = "153--160",
journal = "Bone Marrow Transplantation",
issn = "0268-3369",
publisher = "Nature Publishing Group",
number = "2",

}

TY - JOUR

T1 - Human herpes virus 8 (KSHV) contamination of peripheral blood and autograft products from multiple myeloma patients

AU - Vescio, R. A.

AU - Wu, C. H.

AU - Zheng, L.

AU - Sheen, D.

AU - Ma, H.

AU - Liu, J.

AU - Stewart, A. K.

AU - Ballester, O.

AU - Noga, S. J.

AU - Rugo, H.

AU - Freytes, C.

AU - Stadtmauer, E.

AU - Sahebi, F.

AU - Tarantolo, S.

AU - Stiff, P.

AU - Schiller, G. J.

AU - White, M.

AU - Jacobs, C.

AU - Dipersio, J.

AU - Anderson, K. C.

AU - Berenson, J. R.

PY - 2000

Y1 - 2000

N2 - Human herpesvirus 8 (HHV-8), also known as Kaposi's sarcoma-associated herpesvirus (KSHV), has recently been identified within the bone marrow dendritic cells of multiple myeloma (MM) patients. This virus contains homologues to human cytokines such as IL-6 that could potentially stimulate myeloma cell growth and contribute to disease pathogenesis. Since mobilization chemotherapy may increase circulating dendritic cell numbers, we searched for HHV-8 in peripheral blood mononuclear cells (PBMCs) before and after mobilization chemotherapy given to MM patients. Furthermore, we determined if autograft purging using the CEPRATE SC device would reduce the percentage of HHV-8 infected stem cell products. Only two of the 39 PBMC samples collected prior to mobilization chemotherapy contained PCR detectable virus, yet nine of 37 PBMCs collected on the first day of leukapheresis had detectable HHV-8 (P = 0.016). HHV-8 was more frequently identified in autograft products before vs after Ceprate SC selection (40% vs 15%, P = 0.016). Although the role HHV-8 plays in myeloma pathogenesis remains unclear, these results imply that mobilization chemotherapy increases the numbers of circulating HHV-8-infected dendritic cells within the peripheral blood. In addition, CD34 selection of autograft products in MM patients may reduce the reintroduction of virally infected cells following high-dose chemotherapy.

AB - Human herpesvirus 8 (HHV-8), also known as Kaposi's sarcoma-associated herpesvirus (KSHV), has recently been identified within the bone marrow dendritic cells of multiple myeloma (MM) patients. This virus contains homologues to human cytokines such as IL-6 that could potentially stimulate myeloma cell growth and contribute to disease pathogenesis. Since mobilization chemotherapy may increase circulating dendritic cell numbers, we searched for HHV-8 in peripheral blood mononuclear cells (PBMCs) before and after mobilization chemotherapy given to MM patients. Furthermore, we determined if autograft purging using the CEPRATE SC device would reduce the percentage of HHV-8 infected stem cell products. Only two of the 39 PBMC samples collected prior to mobilization chemotherapy contained PCR detectable virus, yet nine of 37 PBMCs collected on the first day of leukapheresis had detectable HHV-8 (P = 0.016). HHV-8 was more frequently identified in autograft products before vs after Ceprate SC selection (40% vs 15%, P = 0.016). Although the role HHV-8 plays in myeloma pathogenesis remains unclear, these results imply that mobilization chemotherapy increases the numbers of circulating HHV-8-infected dendritic cells within the peripheral blood. In addition, CD34 selection of autograft products in MM patients may reduce the reintroduction of virally infected cells following high-dose chemotherapy.

KW - Autologous transplantation

KW - Human herpesvirus 8

KW - Kaposi's sarcoma-associated herpesvirus

KW - Leukapheresis

KW - Multiple myeloma

KW - Pathogenesis

UR - http://www.scopus.com/inward/record.url?scp=0033621555&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033621555&partnerID=8YFLogxK

M3 - Article

C2 - 10673673

AN - SCOPUS:0033621555

VL - 25

SP - 153

EP - 160

JO - Bone Marrow Transplantation

JF - Bone Marrow Transplantation

SN - 0268-3369

IS - 2

ER -