Human connexin31.9, unlike its orthologous protein connexin30.2 in the mouse, is not detectable in the human cardiac conduction system

Maria M. Kreuzberg, Marcus Liebermann, Sara Segschneider, Radoslaw Dobrowolski, Halina Dobrzynski, Riyaz Kaba, Giselle Rowlinson, Emmanuel Dupont, Nicholas J. Severs, Klaus Willecke

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

In the human heart connexin(Cx)40, Cx43 and Cx45-containing gap junctional channels electrically couple cardiomyocytes, forming a functional syncytium. In the mouse heart, additionally, Cx30.2-containing gap junctions have been detected in the atrioventricular node where they are implicated, together with Cx45, in impulse delay. However, whether the human ortholog of Cx30.2, Cx31.9, is expressed in the human heart has not previously been investigated. We therefore generated Cx31.9 specific antibodies to test for the expression of Cx31.9 in the human heart. These antibodies recognized the Cx31.9 protein in HeLaCx31.9 transfectants by immunofluorescence and immunoblot analyses. However, we did not find punctate Cx31.9 specific immunofluorescence signals in the working myocardium or in the impulse generation and conduction system of adult or fetal human heart. Complementary immunoblot analyses did not reveal Cx31.9 protein in the adult atrial or ventricular myocardium. We conclude that the Cx31.9 protein, unlike its counterpart in the mouse, is not expressed in detectable quantities and is thus unlikely to contribute to the impulse generation and conduction system or the working myocardium of the human heart.

Original languageEnglish (US)
Pages (from-to)553-559
Number of pages7
JournalJournal of Molecular and Cellular Cardiology
Volume46
Issue number4
DOIs
StatePublished - Apr 2009

Keywords

  • Atrioventricular node
  • Cardiac conduction
  • Connexins
  • Cx31.9
  • Electrical coupling
  • GJD3
  • Gap junctions
  • Sinoatrial node

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

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    Kreuzberg, M. M., Liebermann, M., Segschneider, S., Dobrowolski, R., Dobrzynski, H., Kaba, R., Rowlinson, G., Dupont, E., Severs, N. J., & Willecke, K. (2009). Human connexin31.9, unlike its orthologous protein connexin30.2 in the mouse, is not detectable in the human cardiac conduction system. Journal of Molecular and Cellular Cardiology, 46(4), 553-559. https://doi.org/10.1016/j.yjmcc.2008.12.007