Human colony stimulating factor-1 receptor activates the C-raf-1 proto-oncogene kinase

Goutam Ghosh-choudhury, Victor L. Sylvia, Andrea Pfeifer, Ling Mei Wang, Elizabeth Anne Smith, Alan Y Sakaguchi

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The proto-oncogene c-raf-1 encodes a 74 kD serine/threonine kinase. Recently, it has been shown that Raf kinase activity is stimulated by platelet derived growth factor (PDGF) treatment of receptor bearing cells, and that p74 is a direct substrate for PDGF receptor. CSF-1 treatment of BeWo cells, a human choriocarcinoma cell line, and mouse NIH 3T3 cells expressing a transfected human CSF-1 receptor cDNA, was associated with a 3-4 fold increase in phosphorylation of a 74 kD protein immunoprecipitated with affinity purified Raf-1 antibody. The kinase activity of p74 was increased 2-3 fold against two exogenous substrates following CSF-1 treatment of the transfected cells. These observations suggest that Raf-1 protein is a downstream second messenger molecule in CSF-1 mediated signal transduction.

Original languageEnglish (US)
Pages (from-to)154-159
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume172
Issue number1
DOIs
StatePublished - Oct 15 1990

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Colony-Stimulating Factor Receptors
Macrophage Colony-Stimulating Factor
Proto-Oncogenes
Platelet-Derived Growth Factor Receptors
Phosphotransferases
Cells
Bearings (structural)
raf Kinases
Macrophage Colony-Stimulating Factor Receptors
NIH 3T3 Cells
Signal transduction
Choriocarcinoma
Phosphorylation
Protein-Serine-Threonine Kinases
Platelet-Derived Growth Factor
Second Messenger Systems
Substrates
Signal Transduction
Proteins
Complementary DNA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Human colony stimulating factor-1 receptor activates the C-raf-1 proto-oncogene kinase. / Ghosh-choudhury, Goutam; Sylvia, Victor L.; Pfeifer, Andrea; Wang, Ling Mei; Smith, Elizabeth Anne; Sakaguchi, Alan Y.

In: Biochemical and Biophysical Research Communications, Vol. 172, No. 1, 15.10.1990, p. 154-159.

Research output: Contribution to journalArticle

Ghosh-choudhury, Goutam ; Sylvia, Victor L. ; Pfeifer, Andrea ; Wang, Ling Mei ; Smith, Elizabeth Anne ; Sakaguchi, Alan Y. / Human colony stimulating factor-1 receptor activates the C-raf-1 proto-oncogene kinase. In: Biochemical and Biophysical Research Communications. 1990 ; Vol. 172, No. 1. pp. 154-159.
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