TY - JOUR
T1 - Human colony stimulating factor-1 receptor activates the C-raf-1 proto-oncogene kinase
AU - Choudhury, Goutam Ghosh
AU - Sylvia, Victor L.
AU - Pfeifer, Andrea
AU - Wang, Ling Mei
AU - Smith, Elizabeth Anne
AU - Sakaguchi, Alan Y.
N1 - Funding Information:
We thank Mike Bustin for his generous gift of chicken histone H5, Lisa Martinez for technical assistance, Ellen Kraig for use of equipment and Linda Howell for secretarial help. Supported by POI-AG06872 (National Institute on Aging), CD358A (American Cancer Society), and The Meadows Foundation. A.Y.S. dedicates this work to his brother Eckford, a recent victim of cancer.
PY - 1990/10/15
Y1 - 1990/10/15
N2 - The proto-oncogene c-raf-1 encodes a 74 kD serine/threonine kinase. Recently, it has been shown that Raf kinase activity is stimulated by platelet derived growth factor (PDGF) treatment of receptor bearing cells, and that p74 is a direct substrate for PDGF receptor. CSF-1 treatment of BeWo cells, a human choriocarcinoma cell line, and mouse NIH 3T3 cells expressing a transfected human CSF-1 receptor cDNA, was associated with a 3-4 fold increase in phosphorylation of a 74 kD protein immunoprecipitated with affinity purified Raf-1 antibody. The kinase activity of p74 was increased 2-3 fold against two exogenous substrates following CSF-1 treatment of the transfected cells. These observations suggest that Raf-1 protein is a downstream second messenger molecule in CSF-1 mediated signal transduction.
AB - The proto-oncogene c-raf-1 encodes a 74 kD serine/threonine kinase. Recently, it has been shown that Raf kinase activity is stimulated by platelet derived growth factor (PDGF) treatment of receptor bearing cells, and that p74 is a direct substrate for PDGF receptor. CSF-1 treatment of BeWo cells, a human choriocarcinoma cell line, and mouse NIH 3T3 cells expressing a transfected human CSF-1 receptor cDNA, was associated with a 3-4 fold increase in phosphorylation of a 74 kD protein immunoprecipitated with affinity purified Raf-1 antibody. The kinase activity of p74 was increased 2-3 fold against two exogenous substrates following CSF-1 treatment of the transfected cells. These observations suggest that Raf-1 protein is a downstream second messenger molecule in CSF-1 mediated signal transduction.
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U2 - 10.1016/S0006-291X(05)80186-X
DO - 10.1016/S0006-291X(05)80186-X
M3 - Article
C2 - 2222464
AN - SCOPUS:0025084691
VL - 172
SP - 154
EP - 159
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -