Human c-Ki-ras2 proto-oncogene on chromosome 12

Alan Y. Sakaguchi; Susan L. Naylor; Thomas B. Shows; John J. Toole; Melissa McCoy; Robert A. Weinberg

doi:10.1126/science.6823569

Human c-Ki-ras2 proto-oncogene on chromosome 12

Alan Y. Sakaguchi, Susan L. Naylor, Thomas B. Shows, John J. Toole, Melissa McCoy, Robert A. Weinberg

Research output: Contribution to journal › Article › peer-review

59 Scopus citations

Abstract

A human colonic adenocarcinoma transforming gene, recently identified as a cellular homolog of the Kirsten sarcoma gene (ν-ras), was used to assign the human cellular Kirsten ras2 gene to chromosome 12 by the Southern hybridization method. A single 640 base-pair Eco RI-Hind III fragment of the transforming gene, isolated by DNA transfection and molecular cloning, can detect a single Eco RI fragment (2.9 kilobase pairs) of DNA from phenotypically normal cells. The data suggest a constant chromosomal location of c-Ki-ras2.

Original language	English (US)
Pages (from-to)	1081-1083
Number of pages	3
Journal	Science
Volume	219
Issue number	4588
DOIs	https://doi.org/10.1126/science.6823569
State	Published - 1983
Externally published	Yes

ASJC Scopus subject areas

General

Access to Document

10.1126/science.6823569

Cite this

@article{297c07efb19d4cc5a3e4c74aa4a52b65,

title = "Human c-Ki-ras2 proto-oncogene on chromosome 12",

abstract = "A human colonic adenocarcinoma transforming gene, recently identified as a cellular homolog of the Kirsten sarcoma gene (ν-ras), was used to assign the human cellular Kirsten ras2 gene to chromosome 12 by the Southern hybridization method. A single 640 base-pair Eco RI-Hind III fragment of the transforming gene, isolated by DNA transfection and molecular cloning, can detect a single Eco RI fragment (2.9 kilobase pairs) of DNA from phenotypically normal cells. The data suggest a constant chromosomal location of c-Ki-ras2.",

author = "Sakaguchi, \{Alan Y.\} and Naylor, \{Susan L.\} and Shows, \{Thomas B.\} and Toole, \{John J.\} and Melissa McCoy and Weinberg, \{Robert A.\}",

year = "1983",

doi = "10.1126/science.6823569",

language = "English (US)",

volume = "219",

pages = "1081--1083",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "4588",

}

TY - JOUR

T1 - Human c-Ki-ras2 proto-oncogene on chromosome 12

AU - Sakaguchi, Alan Y.

AU - Naylor, Susan L.

AU - Shows, Thomas B.

AU - Toole, John J.

AU - McCoy, Melissa

AU - Weinberg, Robert A.

PY - 1983

Y1 - 1983

N2 - A human colonic adenocarcinoma transforming gene, recently identified as a cellular homolog of the Kirsten sarcoma gene (ν-ras), was used to assign the human cellular Kirsten ras2 gene to chromosome 12 by the Southern hybridization method. A single 640 base-pair Eco RI-Hind III fragment of the transforming gene, isolated by DNA transfection and molecular cloning, can detect a single Eco RI fragment (2.9 kilobase pairs) of DNA from phenotypically normal cells. The data suggest a constant chromosomal location of c-Ki-ras2.

AB - A human colonic adenocarcinoma transforming gene, recently identified as a cellular homolog of the Kirsten sarcoma gene (ν-ras), was used to assign the human cellular Kirsten ras2 gene to chromosome 12 by the Southern hybridization method. A single 640 base-pair Eco RI-Hind III fragment of the transforming gene, isolated by DNA transfection and molecular cloning, can detect a single Eco RI fragment (2.9 kilobase pairs) of DNA from phenotypically normal cells. The data suggest a constant chromosomal location of c-Ki-ras2.

UR - https://www.scopus.com/pages/publications/0020683502

UR - https://www.scopus.com/inward/citedby.url?scp=0020683502&partnerID=8YFLogxK

U2 - 10.1126/science.6823569

DO - 10.1126/science.6823569

M3 - Article

C2 - 6823569

AN - SCOPUS:0020683502

SN - 0036-8075

VL - 219

SP - 1081

EP - 1083

JO - Science

JF - Science

IS - 4588

ER -

Human c-Ki-ras2 proto-oncogene on chromosome 12

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this