Human breast tumor imaging using 111In labeled monoclonal antibody: Athymic mouse model

Ban An Khaw, Joseph S. Bailes, Sandra L. Schneider, Jack Lancaster, James Powers, H. William Strauss, John C. Lasher, William L. McGuire

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

The monoclonal antibody (MoAb) 323/A3, an IgG1, was raised against the human breast tumor cell line MCF-7 and recognized a 43 Kd membrane associated glycoprotein. Histochemical studies with the antibody detected 75% of metastatic lymph nodes, 59% of primary breast tumors, and showed some staining in 20% of benign breast lesions. For radionuclide imaging, the MoAb 323/A3 was labeled with both 125I and 111In, via covalently coupled diethylenetriaminepentaacetic acid (DTPA) by the mixed anhydride method. The antibody activity of the DTPA modified 323/A3 was assessed by an immunoassay using viable and fixed MCF-7 target cells. Male athymic nude mice bearing BT-20 human mammary tumors were injected with dual 125I/111In labeled DTPA 323/A3 via the tail veins. The animals were imaged with a gamma camera equipped with a pinhole collimator at 1-3 h, 1, 2, 3, 4 and 5 days after the tracer administration. On day 5 or 6, the animals were killed, and the biodistribution of the radiotracers was determined for the blood, thyroid, heart, lungs, liver, spleen, kidneys, gastrointestinal tract and tumor. Target to blood ratio at 6 days for the 111In tracer was 24:1 in the group with a mean tumor weight of 0.492 g, and 13:1 in another group with a mean tumor weight of 0.1906 g (day 5). However, the 125I activity showed only 3.6:1 and 5.4:1 target to blood ratios in the corresponding groups. The larger tumors localized less 111In tracer (27.13%±7.57% injected dose/g, Mean±SD) than the smaller tumors (52.75%±22.25% ID/g). Analysis of the gamma images showed that the maximum tracer concentration occurred in the tumors at about 2 to 3 days after intravenous tracer administration. The excellent tumor resolution observed with BT-20 tumors may be due to increased 43 Kd glycoprotein antigen density in this tumor cell line.

Original languageEnglish (US)
Pages (from-to)362-366
Number of pages5
JournalEuropean Journal of Nuclear Medicine
Volume14
Issue number7-8
DOIs
Publication statusPublished - Sep 1 1988

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Keywords

  • Dual radiolabel
  • In
  • Mammary tumor
  • Monoclonal antibody

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Khaw, B. A., Bailes, J. S., Schneider, S. L., Lancaster, J., Powers, J., Strauss, H. W., ... McGuire, W. L. (1988). Human breast tumor imaging using 111In labeled monoclonal antibody: Athymic mouse model. European Journal of Nuclear Medicine, 14(7-8), 362-366. https://doi.org/10.1007/BF00254385