TY - JOUR
T1 - Human antibody responses to a chlamydia-secreted protease factor
AU - Sharma, Jyotika
AU - Bosnie, Anthony M.
AU - Piper, Jeanna M.
AU - Zhong, Guangming
PY - 2004/12
Y1 - 2004/12
N2 - We have previously identified a chlamydia-secreted protein (designated chlamydial proteasome/protease-like activity factor, or CPAF) in the cytosol of chlamydia-infected cells. Although CPAF is known to degrade host transcription factors required for major histocompatibility complex antigen expression in cultured cells, it is not clear whether CPAF is produced and maintains similar functions in humans infected with chlamydial organisms. We now report that CPAF does not preexist in chlamydial organisms and that CPAF synthesis requires live organism replication in cultured cells. Mice inoculated with live, but not mice inoculated with dead, chlamydial organisms produced a strong antibody response to CPAF, correlating CPAF-specific antibody production with CPAF synthesis in animals. Sera from women diagnosed with Chlamydia trachomatis cervicitis displayed higher levels of antibodies to CPAF than to either chlamydial major outer membrane protein or heat shock protein 60, suggesting that CPAF is both produced and immunogenic during human chlamydial infection.
AB - We have previously identified a chlamydia-secreted protein (designated chlamydial proteasome/protease-like activity factor, or CPAF) in the cytosol of chlamydia-infected cells. Although CPAF is known to degrade host transcription factors required for major histocompatibility complex antigen expression in cultured cells, it is not clear whether CPAF is produced and maintains similar functions in humans infected with chlamydial organisms. We now report that CPAF does not preexist in chlamydial organisms and that CPAF synthesis requires live organism replication in cultured cells. Mice inoculated with live, but not mice inoculated with dead, chlamydial organisms produced a strong antibody response to CPAF, correlating CPAF-specific antibody production with CPAF synthesis in animals. Sera from women diagnosed with Chlamydia trachomatis cervicitis displayed higher levels of antibodies to CPAF than to either chlamydial major outer membrane protein or heat shock protein 60, suggesting that CPAF is both produced and immunogenic during human chlamydial infection.
UR - http://www.scopus.com/inward/record.url?scp=9244258599&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=9244258599&partnerID=8YFLogxK
U2 - 10.1128/IAI.72.12.7164-7171.2004
DO - 10.1128/IAI.72.12.7164-7171.2004
M3 - Article
C2 - 15557641
AN - SCOPUS:9244258599
SN - 0019-9567
VL - 72
SP - 7164
EP - 7171
JO - Infection and immunity
JF - Infection and immunity
IS - 12
ER -