Hsp70 dynamics in vivo: Effect of heat shock and protein aggregation

Xian Chun Zeng, Samar Bhasin, Xufeng Wu, Joeng Goo Lee, Shivani Maffi, Christopher J. Nichols, Kyung Jin Lee, J. Paul Taylor, Lois E. Greene, Evan Eisenberg

Research output: Contribution to journalArticlepeer-review

56 Scopus citations

Abstract

The molecular chaperone Hsp70 interacts with misfolded proteins and also accumulates in the nucleus during heat shock. Using GFP-Hsp70 and fluorescence recovery after photobleaching, we show that Hsp70 accumulates in the nucleus during heat shock not only because its inflow rate increases but also because of a marked decrease in its outflow rate. Dynamic imaging also shows that GFP-Hsp70 has greatly reduced mobility when it interacts with organelles such as nucleoli in heat-shocked cells or the large inclusions formed from fragments of mutant huntingtin protein. In heat-shocked cells, nucleoplasmic Hsp70 has reduced mobility relative to the cytoplasm, whereas the ATPase-deficient mutant of Hsp70, Hsp70(K71E), is almost completely immobilized both in the nucleoplasm and the cytoplasm. Moreover, the Hsp70 mutant shows reduced mobility in the presence of diffusive huntingtin fragments with expanded polyglutamine repeats. This provides strong evidence that Hsp70 interacts not only with organelles but also with diffusive proteins in the nucleoplasm and cytoplasm during heat shock as well as with diffusive huntingtin fragments.

Original languageEnglish (US)
Pages (from-to)4991-5000
Number of pages10
JournalJournal of cell science
Volume117
Issue number21
DOIs
StatePublished - Oct 1 2004

Keywords

  • Heat shock
  • Hsp70
  • Huntingtin
  • Mobility

ASJC Scopus subject areas

  • Cell Biology

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