HrcA is a negative regulator of the dnaK and groESL operons of Streptococcus pyogenes

Robyn Woodbury, W. G. Haldenwang

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


The genome of Streptococcus pyogenes, an important human pathogen, encodes homologs of the principal bacterial heat shock proteins DnaK and GroES, -EL, as well as HrcA, a negative regulator of dnaK and groESL expression in other Gram-positive bacteria. Using nuclease protection assays to measure dnaK/groESL mRNA abundance and a "non-polar" insertion to disrupt hrcA, we demonstrate that heat shock triggers a 4- to 8-fold increase in dnaK and groESL-specific mRNAs within 5min of the temperature shift and that HrcA is a negative regulator of S. pyogenes dnaK/groESL mRNA abundance in unstressed S. pyogenes. Although the loss of HrcA elevated dnaK and groESL mRNA levels under non-heat shock conditions, the relative abundance of these RNAs increased further in heat shocked S. pyogenes, suggesting an additional element contributing to their synthesis or stability.

Original languageEnglish (US)
Pages (from-to)722-727
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number4
StatePublished - Mar 21 2003


  • DnaK
  • GroESL
  • Heat shock
  • HrcA
  • Streptococcus pyogenes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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