Hprt activity in embryos of a south american opossum monodelphis domestica

P. G. Johnston, D. Dean, J. L. Vandeberg, E. S. Robinson

    Research output: Contribution to journalArticlepeer-review

    7 Scopus citations


    Marsupial females show preferential paternal X-inactivation. However, the time at which X-inactivation occurs in early development has not yet been determined. A double microassay which measures the activities of X-linked hypoxanthine phosphoribosyl transferase (HPRT) and the autosomally-coded adenine phosphoribosyl transferase (APRT) from the same sample was performed on a collection of embryos from a South American opossum Monodelphis domestica. The embryos ranged in age from the 2-cell stage to the bilaminar blastocyst stage. The results indicate that their embryonic HPRT and APRT are not expressed until just before the unilaminar blastocyst stage in M. domestica. This is at a later stage of development than that in the mouse where embryonic HPRT and APRT expression first occurs at the 4-8-cell stage. It is concluded that HPRT is an uninformative enzyme for assessing X chromosome activity in cleaving embryos of M. domestica. The widespread distribution of HPRT:APRT ratios after the unilaminar blastocyst stage also makes it difficult to draw conclusions about the state of X chromosome activity in early marsupial development.

    Original languageEnglish (US)
    Pages (from-to)529-532
    Number of pages4
    JournalReproduction, Fertility and Development
    Issue number4
    StatePublished - 1994


    • Embyogenesis
    • Marsupials
    • X-inactivation

    ASJC Scopus subject areas

    • Biotechnology
    • Reproductive Medicine
    • Animal Science and Zoology
    • Molecular Biology
    • Genetics
    • Endocrinology
    • Developmental Biology


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