HPRT activity in embryos of a South American opossum Monodelphis domestica.

P. G. Johnston, D. Dean, J. L. VandeBerg, E. S. Robinson

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Marsupial females show preferential paternal X-inactivation. However, the time at which X-inactivation occurs in early development has not yet been determined. A double microassay which measures the activities of X-linked hypoxanthine phosphoribosyl transferase (HPRT) and the autosomally-coded adenine phosphoribosyl transferase (APRT) from the same sample was performed on a collection of embryos from a South American opossum Monodelphis domestica. The embryos ranged in age from the 2-cell stage to the bilaminar blastocyst stage. The results indicate that their embryonic HPRT and APRT are not expressed until just before the unilaminar blastocyst stage in M. domestica. This is at a later stage of development than that in the mouse where embryonic HPRT and APRT expression first occurs at the 4-8-cell stage. It is concluded that HPRT is an uniformative enzyme for assessing X chromosome activity in cleaving embryos of M. domestica. The widespread distribution of HPRT:APRT ratios after the unilaminar blastocyst stage also makes it difficult to draw conclusions about the state of X chromosome activity in early marsupial development.

Original languageEnglish (US)
Pages (from-to)529-532
Number of pages4
JournalReproduction, fertility, and development
Volume6
Issue number4
StatePublished - 1994
Externally publishedYes

Fingerprint

Monodelphis
Monodelphis domestica
Opossums
adenine phosphoribosyltransferase
hypoxanthine
Hypoxanthine
opossums
Transferases
transferases
embryo (animal)
Embryonic Structures
blastocyst
Adenine
X chromosome
Metatheria
Blastocyst
inactivation
Marsupialia
X Chromosome Inactivation
X Chromosome

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Developmental Biology
  • Endocrinology
  • Reproductive Medicine

Cite this

Johnston, P. G., Dean, D., VandeBerg, J. L., & Robinson, E. S. (1994). HPRT activity in embryos of a South American opossum Monodelphis domestica. Reproduction, fertility, and development, 6(4), 529-532.

HPRT activity in embryos of a South American opossum Monodelphis domestica. / Johnston, P. G.; Dean, D.; VandeBerg, J. L.; Robinson, E. S.

In: Reproduction, fertility, and development, Vol. 6, No. 4, 1994, p. 529-532.

Research output: Contribution to journalArticle

Johnston, PG, Dean, D, VandeBerg, JL & Robinson, ES 1994, 'HPRT activity in embryos of a South American opossum Monodelphis domestica.', Reproduction, fertility, and development, vol. 6, no. 4, pp. 529-532.
Johnston, P. G. ; Dean, D. ; VandeBerg, J. L. ; Robinson, E. S. / HPRT activity in embryos of a South American opossum Monodelphis domestica. In: Reproduction, fertility, and development. 1994 ; Vol. 6, No. 4. pp. 529-532.
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