How can we identify parasite genes that underlie antimalarial drug resistance?

Tim Anderson, Standwell Nkhoma, Andrea Ecker, David Fidock

    Research output: Contribution to journalArticle

    40 Scopus citations

    Abstract

    This article outlines genome-scale approaches that can be used to identify mutations in malaria (Plasmodium) parasites that underlie drug resistance and contribute to treatment failure. These approaches include genetic mapping by linkage or genome-wide association studies, drug selection and characterization of resistant mutants, and the identification of genome regions under strong recent selection. While these genomic approaches can identify candidate resistance loci, genetic manipulation is needed to demonstrate causality. We therefore also describe the growing arsenal of available transfection approaches for direct incrimination of mutations suspected to play a role in resistance. Our intention is both to review past progress and highlight promising approaches for future investigations.

    Original languageEnglish (US)
    Pages (from-to)59-85
    Number of pages27
    JournalPharmacogenomics
    Volume12
    Issue number1
    DOIs
    StatePublished - Jan 2011

    Keywords

    • Plasmodium transfection
    • association mapping
    • drug resistance
    • linkage analysis

    ASJC Scopus subject areas

    • Molecular Medicine
    • Genetics
    • Pharmacology

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