Hormone-induced changes in the in vitro DNA-binding activity of the chicken progesterone receptor

R. Rodriguez, M. A. Carson, N. L. Weigel, B. W. O’Malley, W. T. Schrader

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Previous analyses have indicated that steroid hormone receptors undergo an allosteric change in structure upon binding by the steroid ligand. This structural change was envisioned as an intramolecular unmasking of the protein’s DNA-binding domain, thus allowing the receptor to function in gene regulation. We report an analysis of the effect of hormone on the DNA-binding activity of the chicken progesterone receptor. Using an isocratic elution of DNA affinity columns we show that unliganded receptor (aporeceptor) can bind a 23-basepair progesterone response element with high affinity and a high degree of sequence preference. Hormone causes a 1.5-fold increase in affinity for the PRE sequence and a 2-fold decrease in affinity for nonspecific DNA. Kinetic analysis of the off-rate of re-ceptor-DNA complexes is consistent with this minor effect of hormone. In addition, gel retardation analysis of receptor-progesterone response element complexes further substantiates that hormone is not required for sequence-specific DNA binding. These results indicate that hormone is not necessary for the progesterone receptor to fold into a conformation that recognizes specific gene regulatory sequences.

Original languageEnglish (US)
Pages (from-to)356-362
Number of pages7
JournalMolecular Endocrinology
Volume3
Issue number2
DOIs
StatePublished - Feb 1989
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology

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