Hormone-induced and dna demethylation-induced relief of a tissue-specific and developmentally regulated block in transcriptional elongation

Manjeet K. Rao, Yuiko Matsumoto, Marcy E. Richardson, Subbarayalu Panneerdoss, Anjana Bhardwaj, Jacqueline M. Ward, Sreenath Shanker, Anilkumar Bettegowda, Miles F. Wilkinson

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Genome-wide studies have revealed that genes commonly have a high density ofRNApolymerase II just downstream of the transcription start site. This has raised the possibility that genes are commonly regulated by transcriptional elongation, but this remains largely untested in vivo , particularly in vertebrates. Here, we show that the proximal promoter from the Rhox5 homeobox gene recruits polymerase II and begins elongating in all tissues and cell lines that we tested, but it only completes elongation in a tissue-specific and developmentally regulated manner. Relief of the elongation block is associated with recruitment of the elongation factor P-TEFb, the co-activator GRIP1, the chromatin remodeling factor BRG1, and specific histone modifications. We provide evidence that two mechanisms relieve the elongation block at the proximal promoter: demethylation and recruitment of androgen receptor. Together, our findings support a model in which promoter proximal pausing helps confer tissue-specific and developmental gene expression through a mechanism regulated by DNA demethylation-dependent nuclear hormone receptor recruitment.

Original languageEnglish (US)
Pages (from-to)35087-35101
Number of pages15
JournalJournal of Biological Chemistry
Volume289
Issue number51
DOIs
StatePublished - Dec 19 2014

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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