Abstract
A λgt11 library of Mycoplasma genitalium genomic DNA was generated, and clones were identified using a pool of monoclonal antibodies directed against different epitopes of the 140 kDa adhesin protein. Because the 140 kDa protein of M. genitalium and the 170 kDa P1 adhesin of M. pneumoniae share biological properties such as a tip-associated location, cytadherence function and immunologic crossreactivity, we performed Southern blot analysis using these cloned partial 140 kDa gene fragments and 14 subclones that span the P1 structural gene of M. pneumoniae. Homologous regions of the two genes were identified.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 69-73 |
| Number of pages | 5 |
| Journal | Microbial Pathogenesis |
| Volume | 6 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1989 |
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Keywords
- adhesins
- DNA homology
- Mycoplasma
ASJC Scopus subject areas
- Microbiology
- Infectious Diseases
Cite this
Homologous regions shared by adhesin genes of Mycoplasma pneumoniae and Mycoplasma genitalium. / Dallo, Shatha F.; Norton, John R.; Su, Chung Jey; Baseman, Joel B.
In: Microbial Pathogenesis, Vol. 6, No. 1, 1989, p. 69-73.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Homologous regions shared by adhesin genes of Mycoplasma pneumoniae and Mycoplasma genitalium
AU - Dallo, Shatha F.
AU - Norton, John R.
AU - Su, Chung Jey
AU - Baseman, Joel B.
PY - 1989
Y1 - 1989
N2 - A λgt11 library of Mycoplasma genitalium genomic DNA was generated, and clones were identified using a pool of monoclonal antibodies directed against different epitopes of the 140 kDa adhesin protein. Because the 140 kDa protein of M. genitalium and the 170 kDa P1 adhesin of M. pneumoniae share biological properties such as a tip-associated location, cytadherence function and immunologic crossreactivity, we performed Southern blot analysis using these cloned partial 140 kDa gene fragments and 14 subclones that span the P1 structural gene of M. pneumoniae. Homologous regions of the two genes were identified.
AB - A λgt11 library of Mycoplasma genitalium genomic DNA was generated, and clones were identified using a pool of monoclonal antibodies directed against different epitopes of the 140 kDa adhesin protein. Because the 140 kDa protein of M. genitalium and the 170 kDa P1 adhesin of M. pneumoniae share biological properties such as a tip-associated location, cytadherence function and immunologic crossreactivity, we performed Southern blot analysis using these cloned partial 140 kDa gene fragments and 14 subclones that span the P1 structural gene of M. pneumoniae. Homologous regions of the two genes were identified.
KW - adhesins
KW - DNA homology
KW - Mycoplasma
UR - http://www.scopus.com/inward/record.url?scp=0024603903&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024603903&partnerID=8YFLogxK
U2 - 10.1016/0882-4010(89)90009-0
DO - 10.1016/0882-4010(89)90009-0
M3 - Article
C2 - 2499744
AN - SCOPUS:0024603903
VL - 6
SP - 69
EP - 73
JO - Microbial Pathogenesis
JF - Microbial Pathogenesis
SN - 0882-4010
IS - 1
ER -