Homologous regions shared by adhesin genes of Mycoplasma pneumoniae and Mycoplasma genitalium

Shatha F. Dallo; John R. Norton; Chung Jey Su; Joel B. Baseman

doi:10.1016/0882-4010(89)90009-0

Homologous regions shared by adhesin genes of Mycoplasma pneumoniae and Mycoplasma genitalium

Shatha F. Dallo, John R. Norton, Chung Jey Su, Joel B. Baseman

Microbiology, Immunology & Molecular Genetics

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

A λgt11 library of Mycoplasma genitalium genomic DNA was generated, and clones were identified using a pool of monoclonal antibodies directed against different epitopes of the 140 kDa adhesin protein. Because the 140 kDa protein of M. genitalium and the 170 kDa P1 adhesin of M. pneumoniae share biological properties such as a tip-associated location, cytadherence function and immunologic crossreactivity, we performed Southern blot analysis using these cloned partial 140 kDa gene fragments and 14 subclones that span the P1 structural gene of M. pneumoniae. Homologous regions of the two genes were identified.

Original language	English (US)
Pages (from-to)	69-73
Number of pages	5
Journal	Microbial Pathogenesis
Volume	6
Issue number	1
DOIs	https://doi.org/10.1016/0882-4010(89)90009-0
State	Published - Jan 1989

Keywords

DNA homology
Mycoplasma
adhesins

ASJC Scopus subject areas

Microbiology
Infectious Diseases

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10.1016/0882-4010(89)90009-0

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title = "Homologous regions shared by adhesin genes of Mycoplasma pneumoniae and Mycoplasma genitalium",

abstract = "A λgt11 library of Mycoplasma genitalium genomic DNA was generated, and clones were identified using a pool of monoclonal antibodies directed against different epitopes of the 140 kDa adhesin protein. Because the 140 kDa protein of M. genitalium and the 170 kDa P1 adhesin of M. pneumoniae share biological properties such as a tip-associated location, cytadherence function and immunologic crossreactivity, we performed Southern blot analysis using these cloned partial 140 kDa gene fragments and 14 subclones that span the P1 structural gene of M. pneumoniae. Homologous regions of the two genes were identified.",

keywords = "DNA homology, Mycoplasma, adhesins",

author = "Dallo, {Shatha F.} and Norton, {John R.} and Su, {Chung Jey} and Baseman, {Joel B.}",

note = "Funding Information: This study was supported in part by Public Health Service Grant Al 18540 from the National Institute of Allergy and Infectious Diseases .",

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AU - Dallo, Shatha F.

AU - Norton, John R.

AU - Su, Chung Jey

AU - Baseman, Joel B.

N1 - Funding Information: This study was supported in part by Public Health Service Grant Al 18540 from the National Institute of Allergy and Infectious Diseases .

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N2 - A λgt11 library of Mycoplasma genitalium genomic DNA was generated, and clones were identified using a pool of monoclonal antibodies directed against different epitopes of the 140 kDa adhesin protein. Because the 140 kDa protein of M. genitalium and the 170 kDa P1 adhesin of M. pneumoniae share biological properties such as a tip-associated location, cytadherence function and immunologic crossreactivity, we performed Southern blot analysis using these cloned partial 140 kDa gene fragments and 14 subclones that span the P1 structural gene of M. pneumoniae. Homologous regions of the two genes were identified.

AB - A λgt11 library of Mycoplasma genitalium genomic DNA was generated, and clones were identified using a pool of monoclonal antibodies directed against different epitopes of the 140 kDa adhesin protein. Because the 140 kDa protein of M. genitalium and the 170 kDa P1 adhesin of M. pneumoniae share biological properties such as a tip-associated location, cytadherence function and immunologic crossreactivity, we performed Southern blot analysis using these cloned partial 140 kDa gene fragments and 14 subclones that span the P1 structural gene of M. pneumoniae. Homologous regions of the two genes were identified.

KW - DNA homology

KW - Mycoplasma

KW - adhesins

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Homologous regions shared by adhesin genes of Mycoplasma pneumoniae and Mycoplasma genitalium

Abstract

Keywords

ASJC Scopus subject areas

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