Homologous regions shared by adhesin genes of Mycoplasma pneumoniae and Mycoplasma genitalium

Shatha F. Dallo, John R. Norton, Chung Jey Su, Joel B. Baseman

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A λgt11 library of Mycoplasma genitalium genomic DNA was generated, and clones were identified using a pool of monoclonal antibodies directed against different epitopes of the 140 kDa adhesin protein. Because the 140 kDa protein of M. genitalium and the 170 kDa P1 adhesin of M. pneumoniae share biological properties such as a tip-associated location, cytadherence function and immunologic crossreactivity, we performed Southern blot analysis using these cloned partial 140 kDa gene fragments and 14 subclones that span the P1 structural gene of M. pneumoniae. Homologous regions of the two genes were identified.

Original languageEnglish (US)
Pages (from-to)69-73
Number of pages5
JournalMicrobial Pathogenesis
Volume6
Issue number1
DOIs
StatePublished - 1989

Fingerprint

Mycoplasma genitalium
Genes
Mycoplasma pneumoniae
Southern Blotting
Libraries
Epitopes
Proteins
Clone Cells
Monoclonal Antibodies
DNA
Mycoplasma pneumoniae adhesin

Keywords

  • adhesins
  • DNA homology
  • Mycoplasma

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Cite this

Homologous regions shared by adhesin genes of Mycoplasma pneumoniae and Mycoplasma genitalium. / Dallo, Shatha F.; Norton, John R.; Su, Chung Jey; Baseman, Joel B.

In: Microbial Pathogenesis, Vol. 6, No. 1, 1989, p. 69-73.

Research output: Contribution to journalArticle

Dallo, Shatha F. ; Norton, John R. ; Su, Chung Jey ; Baseman, Joel B. / Homologous regions shared by adhesin genes of Mycoplasma pneumoniae and Mycoplasma genitalium. In: Microbial Pathogenesis. 1989 ; Vol. 6, No. 1. pp. 69-73.
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