Holo-TFIID supports transcriptional stimulation by diverse activators and from a TATA-less promoter

Qiang Zhou, Paul M. Lieberman, Thomas G Boyer, Arnold J. Berk

Research output: Contribution to journalArticle

272 Citations (Scopus)

Abstract

Transcription factor HD (TFIID) binds to TATA boxes, nucleating the assembly of initiation complexes containing several general transcription factors and RNA polymerase II. Recently, TFIID was shown to be a multisubunit complex containing a TATA box-binding polypeptide (TBF) and several tightly associated polypeptides (TAFs), which are required for transcriptional stimulation by activator proteins. Here, we report the development of a human cell line expressing an epitope-tagged TBP and the immunopurification of a native, high-molecular-weight form of TFIID that supports transcriptional stimulation by several different classes of activation domains. Recovery of basal and activated TFIID transcriptional specific activity was close to ∼100%. Electrophoretic mobility-shift analysis demonstrated a single major DNA-protein complex. This holo-TFIID contains TAFs of -250, 125, 95, 78, and 50 kD and sediments at 17S. Holo-TFIID produced an extended footprint over the adenovirus major late promoter TATA box and initiator sequence and supported transcriptional activation from a promoter lacking a TATA box. These results lead us to hypothesize that a single multisubunit TFIID protein supports transcriptional stimulation by diverse activation domains and from a TATA-less promoter.

Original languageEnglish (US)
Pages (from-to)1964-1974
Number of pages11
JournalGenes and Development
Volume6
Issue number10
StatePublished - 1992
Externally publishedYes

Fingerprint

Transcription Factor TFIID
TATA Box
General Transcription Factors
Peptides
Proteins
RNA Polymerase II
Human Development
Adenoviridae
Transcriptional Activation
Epitopes
Molecular Weight
Cell Line
DNA

Keywords

  • TATA box
  • TATA-less promoter
  • TFIID
  • Transcriptional activation

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Holo-TFIID supports transcriptional stimulation by diverse activators and from a TATA-less promoter. / Zhou, Qiang; Lieberman, Paul M.; Boyer, Thomas G; Berk, Arnold J.

In: Genes and Development, Vol. 6, No. 10, 1992, p. 1964-1974.

Research output: Contribution to journalArticle

Zhou, Qiang ; Lieberman, Paul M. ; Boyer, Thomas G ; Berk, Arnold J. / Holo-TFIID supports transcriptional stimulation by diverse activators and from a TATA-less promoter. In: Genes and Development. 1992 ; Vol. 6, No. 10. pp. 1964-1974.
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