HIV gp120-specific cell-mediated immune responses in mice after oral immunization with recombinant Salmonella

R. E. Berggren, A. Wunderlich, E. Ziegler, M. Schleicher, R. C. Duke, D. Looney, F. C. Fang

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

Salmonella is of great interest as a potential human immunodeficiency virus vaccine vector because of its ability to elicit potent mucosal and systemic immune responses when administered orally. To determine whether such a vaccine could elicit an immune response in mice, plasmids expressing HIV gp120-LAI were introduced into attenuated S. typhimurium. Three serial doses of 1010 recombinant organisms were administered orally to BALB/c mice at 2- week intervals. Immunized mice but not control mice demonstrated proliferative T cell responses to gp120-LAI, comparable in magnitude to the proliferative responses to Salmonella antigens. Immunized mice had detectable serum and intestinal Salmonella-specific IgA and serum Salmonella-specific IgG. However, no gp120-specific antibody was detected in either serum or intestinal washes. These results indicate that live recombinant Salmonella- based vaccine constructs can induce HIV-specific cellular immune responses in vivo.

Original languageEnglish (US)
Pages (from-to)489-495
Number of pages7
JournalJournal of Acquired Immune Deficiency Syndromes and Human Retrovirology
Volume10
Issue number5
DOIs
StatePublished - 1995
Externally publishedYes

Keywords

  • AIDS
  • ELISA
  • Envelope
  • HIV
  • Mucosal immunization
  • Oral vaccine
  • Recombinant Salmonella
  • Specific T lymphocyte proliferation
  • gp120

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Virology

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