Abstract
Salmonella is of great interest as a potential human immunodeficiency virus vaccine vector because of its ability to elicit potent mucosal and systemic immune responses when administered orally. To determine whether such a vaccine could elicit an immune response in mice, plasmids expressing HIV gp120-LAI were introduced into attenuated S. typhimurium. Three serial doses of 1010 recombinant organisms were administered orally to BALB/c mice at 2- week intervals. Immunized mice but not control mice demonstrated proliferative T cell responses to gp120-LAI, comparable in magnitude to the proliferative responses to Salmonella antigens. Immunized mice had detectable serum and intestinal Salmonella-specific IgA and serum Salmonella-specific IgG. However, no gp120-specific antibody was detected in either serum or intestinal washes. These results indicate that live recombinant Salmonella- based vaccine constructs can induce HIV-specific cellular immune responses in vivo.
Original language | English (US) |
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Pages (from-to) | 489-495 |
Number of pages | 7 |
Journal | Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology |
Volume | 10 |
Issue number | 5 |
DOIs | |
State | Published - 1995 |
Externally published | Yes |
Keywords
- AIDS
- ELISA
- Envelope
- HIV
- Mucosal immunization
- Oral vaccine
- Recombinant Salmonella
- Specific T lymphocyte proliferation
- gp120
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Virology