Abstract
The Tat protein of HIV-1 trans-activates transcription in vitro in a cell-free extract of HeLa nuclei. Quantitative analysis of the efficiency of elongation revealed that a majority of the elongation complexes generated by the HIV-1 promoter were not highly processive and terminated within the first 500 nucleotides. Tat trans-activation of transcription from the HIV-1 promoter resulted from an increase in processive character of the elongation complexes. More specifically, the analysis suggests that there exist two classes of elongation complexes initiating from the HIV promoter: a lessprocessive form and a more-processive form. Addition of purified Tat protein was found to increase the abundance of the more-processive class of elongation complex. The purine nucleoside analog, 5,6-dichloro1-β-D-ribofuranosylbenzimidazole (DRB) inhibits transcription in this reaction by decreasing the efficiency of elongation. Surprisingly, stimulation of transcription elongation by Tat was preferentially inhibited by the addition of DRB.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 4189-4196 |
| Number of pages | 8 |
| Journal | EMBO Journal |
| Volume | 10 |
| Issue number | 13 |
| State | Published - 1991 |
| Externally published | Yes |
Fingerprint
Keywords
- HIV-1
- RNA binding proteins
- TAR
- Tat
- Transcription elongation
ASJC Scopus subject areas
- Cell Biology
- Genetics
Cite this
HIV-1 Tat protein promotes formation of more-processive elongation complexes. / Marciniak, Robert A; Sharp, Phillip A.
In: EMBO Journal, Vol. 10, No. 13, 1991, p. 4189-4196.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - HIV-1 Tat protein promotes formation of more-processive elongation complexes
AU - Marciniak, Robert A
AU - Sharp, Phillip A.
PY - 1991
Y1 - 1991
N2 - The Tat protein of HIV-1 trans-activates transcription in vitro in a cell-free extract of HeLa nuclei. Quantitative analysis of the efficiency of elongation revealed that a majority of the elongation complexes generated by the HIV-1 promoter were not highly processive and terminated within the first 500 nucleotides. Tat trans-activation of transcription from the HIV-1 promoter resulted from an increase in processive character of the elongation complexes. More specifically, the analysis suggests that there exist two classes of elongation complexes initiating from the HIV promoter: a lessprocessive form and a more-processive form. Addition of purified Tat protein was found to increase the abundance of the more-processive class of elongation complex. The purine nucleoside analog, 5,6-dichloro1-β-D-ribofuranosylbenzimidazole (DRB) inhibits transcription in this reaction by decreasing the efficiency of elongation. Surprisingly, stimulation of transcription elongation by Tat was preferentially inhibited by the addition of DRB.
AB - The Tat protein of HIV-1 trans-activates transcription in vitro in a cell-free extract of HeLa nuclei. Quantitative analysis of the efficiency of elongation revealed that a majority of the elongation complexes generated by the HIV-1 promoter were not highly processive and terminated within the first 500 nucleotides. Tat trans-activation of transcription from the HIV-1 promoter resulted from an increase in processive character of the elongation complexes. More specifically, the analysis suggests that there exist two classes of elongation complexes initiating from the HIV promoter: a lessprocessive form and a more-processive form. Addition of purified Tat protein was found to increase the abundance of the more-processive class of elongation complex. The purine nucleoside analog, 5,6-dichloro1-β-D-ribofuranosylbenzimidazole (DRB) inhibits transcription in this reaction by decreasing the efficiency of elongation. Surprisingly, stimulation of transcription elongation by Tat was preferentially inhibited by the addition of DRB.
KW - HIV-1
KW - RNA binding proteins
KW - TAR
KW - Tat
KW - Transcription elongation
UR - http://www.scopus.com/inward/record.url?scp=0026064723&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026064723&partnerID=8YFLogxK
M3 - Article
VL - 10
SP - 4189
EP - 4196
JO - EMBO Journal
JF - EMBO Journal
SN - 0261-4189
IS - 13
ER -