HIV-1 infection of cultured human adult oligodendrocytes

Andrew V. Albright, Julie Strizki, Janet M. Harouse, Ehud Lavi, Michael O'Connor, Francisco González-Scarano

Research output: Contribution to journalArticle

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Abstract

The mechanism through which HIV-1 causes HIV dementia (HIVD) is not well understood. Myelin pallor is a common pathological finding in HIVD and could be explained by a direct infection of oligodendrocytes or interaction with HIV-1 gp 120. To determine if oligodendrocytes could be infected by HIV-1, we purified oligodendrocytes from adult human brain tissue obtained from temporal lobe resections. These cells were exposed to HIV-1 and infectivity was assayed by detection of p24(gag) antigen, PCR amplification, and cocultivation with CD4+ cells. These results indicate that HIV-1(IIIB and BaL) and one of four primary isolates tested can infect oligodendrocytes, resulting in the production of infectious virus. Furthermore, in an experiment that mimics a potential in vivo scenario, infected microglia were able to transmit virus to oligodendrocytes in a trans-well culture system. These experiments indicate that oligodendrocyte infection should be considered in studying the pathophysiology of HIVD.

Original languageEnglish (US)
Pages (from-to)211-219
Number of pages9
JournalVirology
Volume217
Issue number1
DOIs
StatePublished - Mar 1 1996
Externally publishedYes

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Oligodendroglia
HIV Infections
HIV-1
AIDS Dementia Complex
Viruses
Pallor
gag Gene Products
Microglia
Temporal Lobe
Myelin Sheath
Coculture Techniques
Infection
Polymerase Chain Reaction
Brain

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Albright, A. V., Strizki, J., Harouse, J. M., Lavi, E., O'Connor, M., & González-Scarano, F. (1996). HIV-1 infection of cultured human adult oligodendrocytes. Virology, 217(1), 211-219. https://doi.org/10.1006/viro.1996.0108

HIV-1 infection of cultured human adult oligodendrocytes. / Albright, Andrew V.; Strizki, Julie; Harouse, Janet M.; Lavi, Ehud; O'Connor, Michael; González-Scarano, Francisco.

In: Virology, Vol. 217, No. 1, 01.03.1996, p. 211-219.

Research output: Contribution to journalArticle

Albright, AV, Strizki, J, Harouse, JM, Lavi, E, O'Connor, M & González-Scarano, F 1996, 'HIV-1 infection of cultured human adult oligodendrocytes', Virology, vol. 217, no. 1, pp. 211-219. https://doi.org/10.1006/viro.1996.0108
Albright AV, Strizki J, Harouse JM, Lavi E, O'Connor M, González-Scarano F. HIV-1 infection of cultured human adult oligodendrocytes. Virology. 1996 Mar 1;217(1):211-219. https://doi.org/10.1006/viro.1996.0108
Albright, Andrew V. ; Strizki, Julie ; Harouse, Janet M. ; Lavi, Ehud ; O'Connor, Michael ; González-Scarano, Francisco. / HIV-1 infection of cultured human adult oligodendrocytes. In: Virology. 1996 ; Vol. 217, No. 1. pp. 211-219.
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