History and genomic sequence analysis of the herpes simplex virus 1 KOS and KOS1.1 sub-strains

Robert C. Colgrove, Xueqiao Liu, Anthony Griffiths, Priya Raja, Neal A. Deluca, Ruchi M. Newman, Donald M. Coen, David M. Knipe

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A collection of genomic DNA sequences of herpes simplex virus (HSV) strains has been defined and analyzed, and some information is available about genomic stability upon limited passage of viruses in culture. The nature of genomic change upon extensive laboratory passage remains to be determined. In this report we review the history of the HSV-1 KOS laboratory strain and the related KOS1.1 laboratory sub-strain, also called KOS (M), and determine the complete genomic sequence of an early passage stock of the KOS laboratory sub-strain and a laboratory stock of the KOS1.1 sub-strain. The genomes of the two sub-strains are highly similar with only five coding changes, 20 non-coding changes, and about twenty non-ORF sequence changes. The coding changes could potentially explain the KOS1.1 phenotypic properties of increased replication at high temperature and reduced neuroinvasiveness. The study also provides sequence markers to define the provenance of specific laboratory KOS virus stocks.

Original languageEnglish (US)
Pages (from-to)215-221
Number of pages7
JournalVirology
Volume487
DOIs
StatePublished - Jan 1 2016
Externally publishedYes

Fingerprint

Human Herpesvirus 1
Sequence Analysis
History
Viruses
Genomic Instability
Simplexvirus
Genome
Temperature

Keywords

  • Herpes simplex virus
  • HSV
  • HSV genome sequence
  • HSV history
  • KOS
  • KOS sequence
  • KOS1.1
  • KOS1.1sequence

ASJC Scopus subject areas

  • Virology

Cite this

Colgrove, R. C., Liu, X., Griffiths, A., Raja, P., Deluca, N. A., Newman, R. M., ... Knipe, D. M. (2016). History and genomic sequence analysis of the herpes simplex virus 1 KOS and KOS1.1 sub-strains. Virology, 487, 215-221. https://doi.org/10.1016/j.virol.2015.09.026

History and genomic sequence analysis of the herpes simplex virus 1 KOS and KOS1.1 sub-strains. / Colgrove, Robert C.; Liu, Xueqiao; Griffiths, Anthony; Raja, Priya; Deluca, Neal A.; Newman, Ruchi M.; Coen, Donald M.; Knipe, David M.

In: Virology, Vol. 487, 01.01.2016, p. 215-221.

Research output: Contribution to journalArticle

Colgrove, RC, Liu, X, Griffiths, A, Raja, P, Deluca, NA, Newman, RM, Coen, DM & Knipe, DM 2016, 'History and genomic sequence analysis of the herpes simplex virus 1 KOS and KOS1.1 sub-strains', Virology, vol. 487, pp. 215-221. https://doi.org/10.1016/j.virol.2015.09.026
Colgrove RC, Liu X, Griffiths A, Raja P, Deluca NA, Newman RM et al. History and genomic sequence analysis of the herpes simplex virus 1 KOS and KOS1.1 sub-strains. Virology. 2016 Jan 1;487:215-221. https://doi.org/10.1016/j.virol.2015.09.026
Colgrove, Robert C. ; Liu, Xueqiao ; Griffiths, Anthony ; Raja, Priya ; Deluca, Neal A. ; Newman, Ruchi M. ; Coen, Donald M. ; Knipe, David M. / History and genomic sequence analysis of the herpes simplex virus 1 KOS and KOS1.1 sub-strains. In: Virology. 2016 ; Vol. 487. pp. 215-221.
@article{0e7ef02f97784f43ba4945f95da5145f,
title = "History and genomic sequence analysis of the herpes simplex virus 1 KOS and KOS1.1 sub-strains",
abstract = "A collection of genomic DNA sequences of herpes simplex virus (HSV) strains has been defined and analyzed, and some information is available about genomic stability upon limited passage of viruses in culture. The nature of genomic change upon extensive laboratory passage remains to be determined. In this report we review the history of the HSV-1 KOS laboratory strain and the related KOS1.1 laboratory sub-strain, also called KOS (M), and determine the complete genomic sequence of an early passage stock of the KOS laboratory sub-strain and a laboratory stock of the KOS1.1 sub-strain. The genomes of the two sub-strains are highly similar with only five coding changes, 20 non-coding changes, and about twenty non-ORF sequence changes. The coding changes could potentially explain the KOS1.1 phenotypic properties of increased replication at high temperature and reduced neuroinvasiveness. The study also provides sequence markers to define the provenance of specific laboratory KOS virus stocks.",
keywords = "Herpes simplex virus, HSV, HSV genome sequence, HSV history, KOS, KOS sequence, KOS1.1, KOS1.1sequence",
author = "Colgrove, {Robert C.} and Xueqiao Liu and Anthony Griffiths and Priya Raja and Deluca, {Neal A.} and Newman, {Ruchi M.} and Coen, {Donald M.} and Knipe, {David M.}",
year = "2016",
month = "1",
day = "1",
doi = "10.1016/j.virol.2015.09.026",
language = "English (US)",
volume = "487",
pages = "215--221",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - History and genomic sequence analysis of the herpes simplex virus 1 KOS and KOS1.1 sub-strains

AU - Colgrove, Robert C.

AU - Liu, Xueqiao

AU - Griffiths, Anthony

AU - Raja, Priya

AU - Deluca, Neal A.

AU - Newman, Ruchi M.

AU - Coen, Donald M.

AU - Knipe, David M.

PY - 2016/1/1

Y1 - 2016/1/1

N2 - A collection of genomic DNA sequences of herpes simplex virus (HSV) strains has been defined and analyzed, and some information is available about genomic stability upon limited passage of viruses in culture. The nature of genomic change upon extensive laboratory passage remains to be determined. In this report we review the history of the HSV-1 KOS laboratory strain and the related KOS1.1 laboratory sub-strain, also called KOS (M), and determine the complete genomic sequence of an early passage stock of the KOS laboratory sub-strain and a laboratory stock of the KOS1.1 sub-strain. The genomes of the two sub-strains are highly similar with only five coding changes, 20 non-coding changes, and about twenty non-ORF sequence changes. The coding changes could potentially explain the KOS1.1 phenotypic properties of increased replication at high temperature and reduced neuroinvasiveness. The study also provides sequence markers to define the provenance of specific laboratory KOS virus stocks.

AB - A collection of genomic DNA sequences of herpes simplex virus (HSV) strains has been defined and analyzed, and some information is available about genomic stability upon limited passage of viruses in culture. The nature of genomic change upon extensive laboratory passage remains to be determined. In this report we review the history of the HSV-1 KOS laboratory strain and the related KOS1.1 laboratory sub-strain, also called KOS (M), and determine the complete genomic sequence of an early passage stock of the KOS laboratory sub-strain and a laboratory stock of the KOS1.1 sub-strain. The genomes of the two sub-strains are highly similar with only five coding changes, 20 non-coding changes, and about twenty non-ORF sequence changes. The coding changes could potentially explain the KOS1.1 phenotypic properties of increased replication at high temperature and reduced neuroinvasiveness. The study also provides sequence markers to define the provenance of specific laboratory KOS virus stocks.

KW - Herpes simplex virus

KW - HSV

KW - HSV genome sequence

KW - HSV history

KW - KOS

KW - KOS sequence

KW - KOS1.1

KW - KOS1.1sequence

UR - http://www.scopus.com/inward/record.url?scp=84946426536&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84946426536&partnerID=8YFLogxK

U2 - 10.1016/j.virol.2015.09.026

DO - 10.1016/j.virol.2015.09.026

M3 - Article

C2 - 26547038

AN - SCOPUS:84946426536

VL - 487

SP - 215

EP - 221

JO - Virology

JF - Virology

SN - 0042-6822

ER -