History and genomic sequence analysis of the herpes simplex virus 1 KOS and KOS1.1 sub-strains

Robert C. Colgrove, Xueqiao Liu, Anthony Griffiths, Priya Raja, Neal A. Deluca, Ruchi M. Newman, Donald M. Coen, David M. Knipe

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

A collection of genomic DNA sequences of herpes simplex virus (HSV) strains has been defined and analyzed, and some information is available about genomic stability upon limited passage of viruses in culture. The nature of genomic change upon extensive laboratory passage remains to be determined. In this report we review the history of the HSV-1 KOS laboratory strain and the related KOS1.1 laboratory sub-strain, also called KOS (M), and determine the complete genomic sequence of an early passage stock of the KOS laboratory sub-strain and a laboratory stock of the KOS1.1 sub-strain. The genomes of the two sub-strains are highly similar with only five coding changes, 20 non-coding changes, and about twenty non-ORF sequence changes. The coding changes could potentially explain the KOS1.1 phenotypic properties of increased replication at high temperature and reduced neuroinvasiveness. The study also provides sequence markers to define the provenance of specific laboratory KOS virus stocks.

Original languageEnglish (US)
Pages (from-to)215-221
Number of pages7
JournalVirology
Volume487
DOIs
StatePublished - Jan 1 2016
Externally publishedYes

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Keywords

  • Herpes simplex virus
  • HSV
  • HSV genome sequence
  • HSV history
  • KOS
  • KOS sequence
  • KOS1.1
  • KOS1.1sequence

ASJC Scopus subject areas

  • Virology

Cite this

Colgrove, R. C., Liu, X., Griffiths, A., Raja, P., Deluca, N. A., Newman, R. M., Coen, D. M., & Knipe, D. M. (2016). History and genomic sequence analysis of the herpes simplex virus 1 KOS and KOS1.1 sub-strains. Virology, 487, 215-221. https://doi.org/10.1016/j.virol.2015.09.026