Histochemical detection of monoamine oxidase and alcohol dehydrogenase activities in the Syrian hamster Harderian glands: existence of a sexual dimorphism

Armando Menendez-Pelaez, Alberto Martinez-Telleria, Jose A. Vega, Carmen Coalla, Russel J. Reiter

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Abstract

Monoamine oxidase (MAO) and alcohol dehydrogenase (AD) activities were studied histochemically in the Syrian hamster Harderian gland using tryptamine as substrate and Nitroblue Tetrazolium as the final electron acceptor. No dark: light-related changes were observed. Male type I secretory cells showed an intense MAO reaction. Female type I cells exhibited a moderate MAO activity. Both male and female glands showed a moderate/intense AD-positive reaction. Male type II cells were lacking MAO and AD activities. MAO activity found in the hamster Harderian glands corresponded mainly to MAO type A since treatment with chlorgyline (0.01, 0.1 and 0.5 mm) totally inhibited it. The possible role of these two enzymes in Harderian gland indolalkylamine metabolism is discussed.

Original languageEnglish (US)
Pages (from-to)125-130
Number of pages6
JournalThe Histochemical Journal
Volume21
Issue number3
DOIs
Publication statusPublished - Mar 1 1989

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ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

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