Histamine and human parathyroid adenoma: Effect on adenosine 3',5'-monophosphate accumulation in vitro

H. E. Abbound, D. Zimmerman, A. J. Edis, H. Heath, T. P. Dousa

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We studied in vitro the presence of histamine and the effect of histamine and its antagonists on cAMP accumulation in parathyroid tissue (parathyroid adenoma or hyperplasia) from patients with primary hyperparathyroidism. Parathyroid adenomatous tissue contained 11.2 ± 2.9 ng histamine/g wet weight (~ 2 x 10-5 M), as determined by a specific radioenzyme assay. Histamine caused a prominent increase in cAMP accumulation in parathyroid tissue slices in a dose-dependent manner, with half-maximal stimulation being achieved at 5 x 10-6 M and maximal stimulation occurring at 10-4 M histamine. The histamine H2 receptor antagonists, cimetidine and metiamide, caused profound inhibition of histamine-stimulated cAMP accumulated in the parathyroid tissue. Pyrilamine, and H1 antagonist, also inhibited histamine-stimulated cAMP accumulation. Isoproterenol, a β-adrenergic agonist, elicited marked elevation of cAMP, and its stimulatory effect was blocked by propranolol, but the effects of histamine on cAMP levels in parathyroid tissue were not blocked by propranolol. Histamine significantly stimulated (an increase of 50%) the release of immunoreactive parathyroid hormone. The present observations demonstrate that parathyroid adenomatous tissue has a relatively high content of histamine, and that histamine causes both the accumulation of cAMP in and the release of immunoreactive parathyroid hormone from this tissue. The effects of antagonists suggest that histamine stimulates cAMP accumulation in the parathyroid adenomatous tissue by an action on both H2 and H1 histamine receptors.

Original languageEnglish (US)
Pages (from-to)276-281
Number of pages6
JournalJournal of Clinical Endocrinology and Metabolism
Volume53
Issue number2
StatePublished - 1981
Externally publishedYes

Fingerprint

Parathyroid Neoplasms
Adenosine
Histamine
Tissue
Histamine Antagonists
Parathyroid Hormone
Propranolol
Metiamide
Histamine Agents
Histamine H2 Antagonists
Pyrilamine
In Vitro Techniques
Histamine H1 Receptors
Adrenergic Agonists
Histamine H2 Receptors
Primary Hyperparathyroidism
Cimetidine
Isoproterenol
Hyperplasia
Assays

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Histamine and human parathyroid adenoma : Effect on adenosine 3',5'-monophosphate accumulation in vitro. / Abbound, H. E.; Zimmerman, D.; Edis, A. J.; Heath, H.; Dousa, T. P.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 53, No. 2, 1981, p. 276-281.

Research output: Contribution to journalArticle

Abbound, H. E. ; Zimmerman, D. ; Edis, A. J. ; Heath, H. ; Dousa, T. P. / Histamine and human parathyroid adenoma : Effect on adenosine 3',5'-monophosphate accumulation in vitro. In: Journal of Clinical Endocrinology and Metabolism. 1981 ; Vol. 53, No. 2. pp. 276-281.
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