High-throughput microarray for antimicrobial susceptibility testing

Anand Srinivasan, Grace C. Lee, Nelson S. Torres, Kevin Hernandez, Steven D. Dallas, Jose Lopez-Ribot, Christopher R. Frei, Anand K. Ramasubramanian

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

We describe the development of a novel, high-throughput, nano-scale microarray platform for antimicrobial susceptibility testing (AST). The platform allows to process 480 samples at 50 nL volume on a single chip, analyze by fluorescence read-out with an easy dunk-and-rinse step, and the ability to process multiple samples and chips simultaneously. We demonstrate the applicability of this chip for culturing community acquired methicillin resistant Staphylococcus aureus (CA-MRSA), and perform AST against clinical isolates of CA-MRSA. The chip platform holds promise for an impact in microbial biotechnology as an attractive high-throughput, lower sample volume and quicker alternative to conventional AST such as the traditional broth microdilution or the newer automated systems.

Original languageEnglish (US)
Pages (from-to)44-47
Number of pages4
JournalBiotechnology Reports
Volume16
DOIs
StatePublished - Dec 2017

Keywords

  • Antimicrobial susceptibility testing
  • High-throughput
  • Microarray

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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    Srinivasan, A., Lee, G. C., Torres, N. S., Hernandez, K., Dallas, S. D., Lopez-Ribot, J., Frei, C. R., & Ramasubramanian, A. K. (2017). High-throughput microarray for antimicrobial susceptibility testing. Biotechnology Reports, 16, 44-47. https://doi.org/10.1016/j.btre.2017.10.004