High-throughput cell transplantation establishes that tumor-initiating cells are abundant in zebrafish T-cell acute lymphoblastic leukemia

Alexandra C.H. Smith, Aubrey R. Raimondi, Chris D. Salthouse, Myron S Ignatius, Jessica S. Blackburn, Igor V. Mizgirev, Narie Y. Storer, Jill L.O. De Jong, Aye T. Chen, Yi Zhou, Sergei Revskoy, Leonard I. Zon, David M. Langenau

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

Self-renewal is a feature of cancer and can be assessed by cell transplantation into immune-compromised or immune-matched animals. However, studies in zebrafish have been severely limited by lack of these reagents. Here, Myc-induced T-cell acute lymphoblastic leukemias (T-ALLs) have been made in syngeneic, clonal zebrafish and can be transplanted into sibling animals without the need for immune suppression. These studies show that self-renewing cells are abundant in T-ALL and comprise 0.1% to 15.9% of the T-ALL mass. Large-scale single-cell transplantation experiments established that T-ALLs can be initiated from a single cell and that leukemias exhibit wide differences in tumor-initiating potential. T-ALLs also can be introduced into clonal-outcrossed animals, and T-ALLs arising in mixed genetic backgrounds can be transplanted into clonal recipients without the need for major histocompatibility complex matching. Finally, high-throughput imaging methods are described that allow large numbers of fluorescent transgenic animals to be imaged simultaneously, facilitating the rapid screening of engrafted animals. Our experiments highlight the large numbers of zebrafish that can be experimentally assessed by cell transplantation and establish new high-throughput methods to functionally interrogate gene pathways involved in cancer self-renewal.

Original languageEnglish (US)
Pages (from-to)3296-3303
Number of pages8
JournalBlood
Volume115
Issue number16
DOIs
StatePublished - Apr 22 2010
Externally publishedYes

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Precursor T-Cell Lymphoblastic Leukemia-Lymphoma
T-cells
Neoplastic Stem Cells
Cell Transplantation
Zebrafish
Tumors
Throughput
Animals
Neoplasms
Genetically Modified Animals
Major Histocompatibility Complex
Screening
Leukemia
Genes
Experiments
Imaging techniques

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Smith, A. C. H., Raimondi, A. R., Salthouse, C. D., Ignatius, M. S., Blackburn, J. S., Mizgirev, I. V., ... Langenau, D. M. (2010). High-throughput cell transplantation establishes that tumor-initiating cells are abundant in zebrafish T-cell acute lymphoblastic leukemia. Blood, 115(16), 3296-3303. https://doi.org/10.1182/blood-2009-10-246488

High-throughput cell transplantation establishes that tumor-initiating cells are abundant in zebrafish T-cell acute lymphoblastic leukemia. / Smith, Alexandra C.H.; Raimondi, Aubrey R.; Salthouse, Chris D.; Ignatius, Myron S; Blackburn, Jessica S.; Mizgirev, Igor V.; Storer, Narie Y.; De Jong, Jill L.O.; Chen, Aye T.; Zhou, Yi; Revskoy, Sergei; Zon, Leonard I.; Langenau, David M.

In: Blood, Vol. 115, No. 16, 22.04.2010, p. 3296-3303.

Research output: Contribution to journalArticle

Smith, ACH, Raimondi, AR, Salthouse, CD, Ignatius, MS, Blackburn, JS, Mizgirev, IV, Storer, NY, De Jong, JLO, Chen, AT, Zhou, Y, Revskoy, S, Zon, LI & Langenau, DM 2010, 'High-throughput cell transplantation establishes that tumor-initiating cells are abundant in zebrafish T-cell acute lymphoblastic leukemia', Blood, vol. 115, no. 16, pp. 3296-3303. https://doi.org/10.1182/blood-2009-10-246488
Smith, Alexandra C.H. ; Raimondi, Aubrey R. ; Salthouse, Chris D. ; Ignatius, Myron S ; Blackburn, Jessica S. ; Mizgirev, Igor V. ; Storer, Narie Y. ; De Jong, Jill L.O. ; Chen, Aye T. ; Zhou, Yi ; Revskoy, Sergei ; Zon, Leonard I. ; Langenau, David M. / High-throughput cell transplantation establishes that tumor-initiating cells are abundant in zebrafish T-cell acute lymphoblastic leukemia. In: Blood. 2010 ; Vol. 115, No. 16. pp. 3296-3303.
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