High-throughput antibody isolation

Andrew Hayhurst, George Georgiou

    Research output: Contribution to journalReview article

    47 Scopus citations

    Abstract

    To define the proteome of an organism, there is a need for robust and reproducible methods for the quantitative detection of all the polypeptides in a cell. High-density arrays of receptors specific for each of the polypeptides in a complex sample hold great promise for the analysis of complex protein mixtures. Because of their high affinity, specificity and their ability to bind to virtually any protein, antibodies appear particularly promising as the receptor element in protein-detection arrays. For proteomic-scale analyses, the ability to isolate and produce antibodies en masse to large numbers of target molecules is critical. A variety of systems for the high-throughput isolation of antibodies from combinatorial libraries are being developed and are outlined in this review. However, there are several other important considerations to be borne in mind before such systems can realistically be applied on a large scale.

    Original languageEnglish (US)
    Pages (from-to)683-689
    Number of pages7
    JournalCurrent Opinion in Chemical Biology
    Volume5
    Issue number6
    DOIs
    StatePublished - Dec 1 2001

    ASJC Scopus subject areas

    • Analytical Chemistry
    • Biochemistry

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