High-resolution three-dimensional microelectrode brain mapping using stereo microfocal X-ray imaging

David D. Cox, Alexander M Papanastassiou, Daniel Oreper, Benjamin B. Andken, James J. DiCarlo

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Much of our knowledge of brain function has been gleaned from studies using microelectrodes to characterize the response properties of individual neurons in vivo. However, because it is difficult to accurately determine the location of a microelectrode tip within the brain, it is impossible to systematically map the fine three-dimensional spatial organization of many brain areas, especially in deep structures. Here, we present a practical method based on digital stereo microfocal X-ray imaging that makes it possible to estimate the three-dimensional position of each and every microelectrode recording site in "real time" during experimental sessions. We determined the system's ex vivo localization accuracy to be better than 50 μm, and we show how we have used this method to coregister hundreds of deep-brain microelectrode recordings in monkeys to a common frame of reference with median error of <150 μm. We further show how we can coregister those sites with magnetic resonance images (MRIs), allowing for comparison with anatomy, and laying the groundwork for more detailed electrophysiology/functional MRI comparison. Minimally, this method allows one to marry the single-cell specificity of microelectrode recording with the spatial mapping abilities of imaging techniques; furthermore, it has the potential of yielding fundamentally new kinds of high-resolution maps of brain function.

Original languageEnglish (US)
Pages (from-to)2966-2976
Number of pages11
JournalJournal of Neurophysiology
Volume100
Issue number5
DOIs
StatePublished - Nov 2008
Externally publishedYes

Fingerprint

Brain Mapping
Microelectrodes
X-Rays
Brain
Magnetic Resonance Spectroscopy
Electrophysiology
Haplorhini
Anatomy
Neurons

ASJC Scopus subject areas

  • Physiology
  • Neuroscience(all)

Cite this

High-resolution three-dimensional microelectrode brain mapping using stereo microfocal X-ray imaging. / Cox, David D.; Papanastassiou, Alexander M; Oreper, Daniel; Andken, Benjamin B.; DiCarlo, James J.

In: Journal of Neurophysiology, Vol. 100, No. 5, 11.2008, p. 2966-2976.

Research output: Contribution to journalArticle

Cox, David D. ; Papanastassiou, Alexander M ; Oreper, Daniel ; Andken, Benjamin B. ; DiCarlo, James J. / High-resolution three-dimensional microelectrode brain mapping using stereo microfocal X-ray imaging. In: Journal of Neurophysiology. 2008 ; Vol. 100, No. 5. pp. 2966-2976.
@article{2a41b3804f4448a9b63b7662ee7ff9d3,
title = "High-resolution three-dimensional microelectrode brain mapping using stereo microfocal X-ray imaging",
abstract = "Much of our knowledge of brain function has been gleaned from studies using microelectrodes to characterize the response properties of individual neurons in vivo. However, because it is difficult to accurately determine the location of a microelectrode tip within the brain, it is impossible to systematically map the fine three-dimensional spatial organization of many brain areas, especially in deep structures. Here, we present a practical method based on digital stereo microfocal X-ray imaging that makes it possible to estimate the three-dimensional position of each and every microelectrode recording site in {"}real time{"} during experimental sessions. We determined the system's ex vivo localization accuracy to be better than 50 μm, and we show how we have used this method to coregister hundreds of deep-brain microelectrode recordings in monkeys to a common frame of reference with median error of <150 μm. We further show how we can coregister those sites with magnetic resonance images (MRIs), allowing for comparison with anatomy, and laying the groundwork for more detailed electrophysiology/functional MRI comparison. Minimally, this method allows one to marry the single-cell specificity of microelectrode recording with the spatial mapping abilities of imaging techniques; furthermore, it has the potential of yielding fundamentally new kinds of high-resolution maps of brain function.",
author = "Cox, {David D.} and Papanastassiou, {Alexander M} and Daniel Oreper and Andken, {Benjamin B.} and DiCarlo, {James J.}",
year = "2008",
month = "11",
doi = "10.1152/jn.90672.2008",
language = "English (US)",
volume = "100",
pages = "2966--2976",
journal = "Journal of Neurophysiology",
issn = "0022-3077",
publisher = "American Physiological Society",
number = "5",

}

TY - JOUR

T1 - High-resolution three-dimensional microelectrode brain mapping using stereo microfocal X-ray imaging

AU - Cox, David D.

AU - Papanastassiou, Alexander M

AU - Oreper, Daniel

AU - Andken, Benjamin B.

AU - DiCarlo, James J.

PY - 2008/11

Y1 - 2008/11

N2 - Much of our knowledge of brain function has been gleaned from studies using microelectrodes to characterize the response properties of individual neurons in vivo. However, because it is difficult to accurately determine the location of a microelectrode tip within the brain, it is impossible to systematically map the fine three-dimensional spatial organization of many brain areas, especially in deep structures. Here, we present a practical method based on digital stereo microfocal X-ray imaging that makes it possible to estimate the three-dimensional position of each and every microelectrode recording site in "real time" during experimental sessions. We determined the system's ex vivo localization accuracy to be better than 50 μm, and we show how we have used this method to coregister hundreds of deep-brain microelectrode recordings in monkeys to a common frame of reference with median error of <150 μm. We further show how we can coregister those sites with magnetic resonance images (MRIs), allowing for comparison with anatomy, and laying the groundwork for more detailed electrophysiology/functional MRI comparison. Minimally, this method allows one to marry the single-cell specificity of microelectrode recording with the spatial mapping abilities of imaging techniques; furthermore, it has the potential of yielding fundamentally new kinds of high-resolution maps of brain function.

AB - Much of our knowledge of brain function has been gleaned from studies using microelectrodes to characterize the response properties of individual neurons in vivo. However, because it is difficult to accurately determine the location of a microelectrode tip within the brain, it is impossible to systematically map the fine three-dimensional spatial organization of many brain areas, especially in deep structures. Here, we present a practical method based on digital stereo microfocal X-ray imaging that makes it possible to estimate the three-dimensional position of each and every microelectrode recording site in "real time" during experimental sessions. We determined the system's ex vivo localization accuracy to be better than 50 μm, and we show how we have used this method to coregister hundreds of deep-brain microelectrode recordings in monkeys to a common frame of reference with median error of <150 μm. We further show how we can coregister those sites with magnetic resonance images (MRIs), allowing for comparison with anatomy, and laying the groundwork for more detailed electrophysiology/functional MRI comparison. Minimally, this method allows one to marry the single-cell specificity of microelectrode recording with the spatial mapping abilities of imaging techniques; furthermore, it has the potential of yielding fundamentally new kinds of high-resolution maps of brain function.

UR - http://www.scopus.com/inward/record.url?scp=57049154588&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=57049154588&partnerID=8YFLogxK

U2 - 10.1152/jn.90672.2008

DO - 10.1152/jn.90672.2008

M3 - Article

VL - 100

SP - 2966

EP - 2976

JO - Journal of Neurophysiology

JF - Journal of Neurophysiology

SN - 0022-3077

IS - 5

ER -