High-Resolution Genomic Mapping of the Three Human Replication Protein A Genes (RPA1, RPA2, and RPA3)

C. B. Umbricht, C. A. Griffin, A. L. Hawkins, K. H. Grzeschik, P. O'Connell, Robin J Leach, E. D. Green, T. J. Kelly

Research output: Contribution to journalArticle

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Abstract

Human replication protein A (RPA) is a three-subunit protein that plays a central role in eukaryotic DNA replication, homologous recombination, and excision repair. We have previously reported the cloning and bacterial overexpression of the three RPA genes and have mapped them to chromosome 1 (RPA2), chromosome 7 (RPA3), and chromosome 17 (RPA1). We have now obtained yeast strains with artificial chromosomes carrying the three human RPA genes and report the more detailed genomic mapping of RPA. RPA1 was mapped to chromosome 17p13.3 using a combination of PCR amplification of somatic cell hybrids and radiation hybrids containing chromosome 17 fragments. RPA2 was mapped to chromosome 1p35 by PCR amplification of somatic cell hybrids of chromosome 1 and by fluorescence in situ hybridization. RPA3 was mapped to chromosome 7p22 by Southern analysis and PCR amplification of somatic cell hybrids of chromosome 7 as well as fluorescence in situ hybridization. Since RPA is an essential component of major metabolic events affecting DNA, the physical mapping of the genes for it may help elucidate the biochemical basis of genetic disorders involving DNA metabolism.

Original languageEnglish (US)
Pages (from-to)249-257
Number of pages9
JournalGenomics
Volume20
Issue number2
DOIs
StatePublished - Mar 15 1994

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vif Genes
Chromosomes, Human, Pair 17
Hybrid Cells
Replication Protein A
Chromosomes, Human, Pair 7
Chromosomes
Chromosomes, Human, Pair 1
Fluorescence In Situ Hybridization
Polymerase Chain Reaction
Artificial Chromosomes
Radiation Hybrid Mapping
Recombinational DNA Repair
Inborn Genetic Diseases
Proteins
Chromosome Mapping
DNA
Protein Subunits
DNA Replication
DNA Repair
Organism Cloning

ASJC Scopus subject areas

  • Genetics

Cite this

Umbricht, C. B., Griffin, C. A., Hawkins, A. L., Grzeschik, K. H., O'Connell, P., Leach, R. J., ... Kelly, T. J. (1994). High-Resolution Genomic Mapping of the Three Human Replication Protein A Genes (RPA1, RPA2, and RPA3). Genomics, 20(2), 249-257. https://doi.org/10.1006/geno.1994.1161

High-Resolution Genomic Mapping of the Three Human Replication Protein A Genes (RPA1, RPA2, and RPA3). / Umbricht, C. B.; Griffin, C. A.; Hawkins, A. L.; Grzeschik, K. H.; O'Connell, P.; Leach, Robin J; Green, E. D.; Kelly, T. J.

In: Genomics, Vol. 20, No. 2, 15.03.1994, p. 249-257.

Research output: Contribution to journalArticle

Umbricht, CB, Griffin, CA, Hawkins, AL, Grzeschik, KH, O'Connell, P, Leach, RJ, Green, ED & Kelly, TJ 1994, 'High-Resolution Genomic Mapping of the Three Human Replication Protein A Genes (RPA1, RPA2, and RPA3)', Genomics, vol. 20, no. 2, pp. 249-257. https://doi.org/10.1006/geno.1994.1161
Umbricht, C. B. ; Griffin, C. A. ; Hawkins, A. L. ; Grzeschik, K. H. ; O'Connell, P. ; Leach, Robin J ; Green, E. D. ; Kelly, T. J. / High-Resolution Genomic Mapping of the Three Human Replication Protein A Genes (RPA1, RPA2, and RPA3). In: Genomics. 1994 ; Vol. 20, No. 2. pp. 249-257.
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