High-performance liquid chromatography of methotrexate analogs containing terminal lysine or ornithine and their dansyl derivatives

A. Ashok Kumar, Robert J. Kempton, Gregory M. Anstead, Elmer M. Price, James H. Freisheim

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

A procedure utilizing a reverse-phase semipreparative high-performance liquid chromatography column and a binary solvent system consisting of trifluoroacetic acid and 1-propanol has been developed for the semipreparative scale purification and analytical identification of four newly synthesized analogs of methotrexate. The methotrexate analogs containing a lysine or an ornithine residue in place of a terminal glutamate residue together with their respective dansyl derivatives were purified in milligram quantities by the procedures described.

Original languageEnglish (US)
Pages (from-to)191-195
Number of pages5
JournalAnalytical Biochemistry
Volume128
Issue number1
DOIs
StatePublished - Jan 1983
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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