High-performance liquid chromatography of methotrexate analogs containing terminal lysine or ornithine and their dansyl derivatives

A. Ashok Kumar; Robert J. Kempton; Gregory M. Anstead; Elmer M. Price; James H. Freisheim

doi:10.1016/0003-2697(83)90362-7

High-performance liquid chromatography of methotrexate analogs containing terminal lysine or ornithine and their dansyl derivatives

A. Ashok Kumar, Robert J. Kempton, Gregory M. Anstead, Elmer M. Price, James H. Freisheim

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

A procedure utilizing a reverse-phase semipreparative high-performance liquid chromatography column and a binary solvent system consisting of trifluoroacetic acid and 1-propanol has been developed for the semipreparative scale purification and analytical identification of four newly synthesized analogs of methotrexate. The methotrexate analogs containing a lysine or an ornithine residue in place of a terminal glutamate residue together with their respective dansyl derivatives were purified in milligram quantities by the procedures described.

Original language	English (US)
Pages (from-to)	191-195
Number of pages	5
Journal	Analytical Biochemistry
Volume	128
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(83)90362-7
State	Published - Jan 1983
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Biophysics
Biochemistry
Cell Biology

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title = "High-performance liquid chromatography of methotrexate analogs containing terminal lysine or ornithine and their dansyl derivatives",

abstract = "A procedure utilizing a reverse-phase semipreparative high-performance liquid chromatography column and a binary solvent system consisting of trifluoroacetic acid and 1-propanol has been developed for the semipreparative scale purification and analytical identification of four newly synthesized analogs of methotrexate. The methotrexate analogs containing a lysine or an ornithine residue in place of a terminal glutamate residue together with their respective dansyl derivatives were purified in milligram quantities by the procedures described.",

author = "Kumar, {A. Ashok} and Kempton, {Robert J.} and Anstead, {Gregory M.} and Price, {Elmer M.} and Freisheim, {James H.}",

note = "Funding Information: This work was supported in part by PHS Grant CA 11666, National Cancer Institute, DHHS (to J.H.F.), and by a grant from the Research Corporation (to R.J.K.). Grateful acknowledgement is made to the Albert and Louise Cooper Scholarship Fund for support of G.M.A. The authors thank Mr. Philip Smith and Mr. Larry Judge for their excellent technical assistance.",

year = "1983",

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doi = "10.1016/0003-2697(83)90362-7",

language = "English (US)",

volume = "128",

pages = "191--195",

journal = "Analytical Biochemistry",

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T1 - High-performance liquid chromatography of methotrexate analogs containing terminal lysine or ornithine and their dansyl derivatives

AU - Kumar, A. Ashok

AU - Kempton, Robert J.

AU - Anstead, Gregory M.

AU - Price, Elmer M.

AU - Freisheim, James H.

N1 - Funding Information: This work was supported in part by PHS Grant CA 11666, National Cancer Institute, DHHS (to J.H.F.), and by a grant from the Research Corporation (to R.J.K.). Grateful acknowledgement is made to the Albert and Louise Cooper Scholarship Fund for support of G.M.A. The authors thank Mr. Philip Smith and Mr. Larry Judge for their excellent technical assistance.

PY - 1983/1

Y1 - 1983/1

N2 - A procedure utilizing a reverse-phase semipreparative high-performance liquid chromatography column and a binary solvent system consisting of trifluoroacetic acid and 1-propanol has been developed for the semipreparative scale purification and analytical identification of four newly synthesized analogs of methotrexate. The methotrexate analogs containing a lysine or an ornithine residue in place of a terminal glutamate residue together with their respective dansyl derivatives were purified in milligram quantities by the procedures described.

AB - A procedure utilizing a reverse-phase semipreparative high-performance liquid chromatography column and a binary solvent system consisting of trifluoroacetic acid and 1-propanol has been developed for the semipreparative scale purification and analytical identification of four newly synthesized analogs of methotrexate. The methotrexate analogs containing a lysine or an ornithine residue in place of a terminal glutamate residue together with their respective dansyl derivatives were purified in milligram quantities by the procedures described.

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High-performance liquid chromatography of methotrexate analogs containing terminal lysine or ornithine and their dansyl derivatives

Abstract

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