High murine blood persistence of phage T3 and suggested strategy for phage therapy

Philip Serwer, Elena T. Wright, John C. Lee

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Objective: Our immediate objective is to determine whether infectivity of lytic podophage T3 has a relatively high persistence in the blood of a mouse, as suggested by previous data. Secondarily, we determine whether the T3 surface has changed during this mouse passage. The surface is characterized by native agarose gel electrophoresis (AGE). Beyond our current data, the long-term objective is optimization of phages chosen for therapy of all bacteremias and associated sepsis. Results: We find that the persistence of T3 in mouse blood is higher by over an order of magnitude than the previously reported persistence of (1) lysogenic phages lambda and P22, and (2) lytic phage T7, a T3 relative. We explain these differences via the lysogenic character of lambda and P22, and the physical properties of T7. For the future, we propose testing a new, AGE-based strategy for rapidly screening for high-persistence, lytic, environmental podophages that have phage therapy-promoting physical properties.

Original languageEnglish (US)
Article number560
JournalBMC Research Notes
Volume12
Issue number1
DOIs
Publication statusPublished - Sep 5 2019

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Keywords

  • Average electrical surface charge density
  • Bacteremia
  • Innate immune systems
  • Pan-antibiotic-resistant bacteria
  • Rapid phage characterization, strategy for

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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