High levels of serum collagenase in premature labor - a potential biochemical marker

M. Rajabi, David D Dean, J. F. Woessner

Research output: Contribution to journalArticle

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Abstract

An improved sensitive assay for collagenase, which uses [3H]telopeptide-free collagen as a substrate, was used to measure changes in serum collagenase levels in 96 women and ten men (18-35 years old). Both latent and active forms of collagenase were detected in serum by molecular sieve chromatography; these forms had a relative molecular weight (M(r)) of 65,000 and 45,000, respectively. Only latent collagenase was detected in crude serum after destroying inhibitors by treatment with 3 M potassium thiocyanate. Collagenase levels in males were lower than in nongravid females (34 ± 5 versus 53 ± 5 U/dL; mean ± SEM; 1 unit = 1 μg collagen digested per minute at 30C). During pregnancy there was no significant change in serum collagenase levels until the onset of spontaneous labor in full-term pregnancies (37-42 weeks), at which point there was a 66% increase over the nongravid level to a value of 88 ± 5 U/dL. There was a further rise at one day postpartum, and high levels continued for at least four days. Women in premature labor (24-36 weeks) exhibited an eightfold increase in the level of serum collagenase to 405 ± 110 U/dL; 16 of 17 patients in this group had collagenase levels above the 95th percentile for women at 16-40 weeks but not in labor. This evaluation of serum collagenase may provide a key for detecting premature labor. It is suggested that the increase in serum collagenase arises from the lower uterine segment and cervix.

Original languageEnglish (US)
Pages (from-to)179-186
Number of pages8
JournalObstetrics and Gynecology
Volume69
Issue number2
StatePublished - 1987
Externally publishedYes

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Premature Obstetric Labor
Collagenases
Biomarkers
Serum
Collagen
Labor Onset
Pregnancy
Cervix Uteri
Postpartum Period
Gel Chromatography
Molecular Weight

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

High levels of serum collagenase in premature labor - a potential biochemical marker. / Rajabi, M.; Dean, David D; Woessner, J. F.

In: Obstetrics and Gynecology, Vol. 69, No. 2, 1987, p. 179-186.

Research output: Contribution to journalArticle

Rajabi, M, Dean, DD & Woessner, JF 1987, 'High levels of serum collagenase in premature labor - a potential biochemical marker', Obstetrics and Gynecology, vol. 69, no. 2, pp. 179-186.
Rajabi, M. ; Dean, David D ; Woessner, J. F. / High levels of serum collagenase in premature labor - a potential biochemical marker. In: Obstetrics and Gynecology. 1987 ; Vol. 69, No. 2. pp. 179-186.
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