High level expression and phosphorylation of hepatitis b virus polymerase in insect cells with recombinant baculoviruses

Brick Ayola, Patrick Kanda, Robert E. Lanford

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

The hepatitis B virus polymerase open reading frame, as well as various subdomains of polymerase, was expressed in insect cells using the recombinant baculovirus expression system. Full-length polymerase was expressed at very low levels in a Spodoptera frugiperda cell line, the amino-terminal domain of polymerase was expressed at high levels, and other constructs were expressed at intermediate levels. Infections of a Trichoplusia ni cell line with the same recombinant baculoviruses resulted in high levels of protein production for all polymerase constructs. Each of the polymerase polypeptides was phosphorylated in insect cells. Since polypeptides with non-overlapping sequences were phosphorylated, polymerase must be phosphorylated at a minimum of two sites.

Original languageEnglish (US)
Pages (from-to)370-373
Number of pages4
JournalVirology
Volume194
Issue number1
DOIs
StatePublished - May 1993
Externally publishedYes

ASJC Scopus subject areas

  • Virology

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