TY - JOUR
T1 - High glucose induced NF-κB DNA-binding activity in HAEC is maintained under low shear stress but inhibited under high shear stress
T2 - Role of nitric oxide
AU - Mohan, Sumathy
AU - Hamuro, Masao
AU - Koyoma, Koichi
AU - Sorescu, George P.
AU - Jo, Hanjoong
AU - Natarajan, Mohan
N1 - Funding Information:
We thank Mr. Javier Chapa, UTHSCSA for technical help in cell culture and shear stress experiments and Dr. Thomas J Prihoda for assistance in statistical analysis. This work was supported by Juvenile Diabetes Foundation International Research Grant (1-2000-203) and RO1 HL63032-01A1from NIH-NHLBI.
PY - 2003/12
Y1 - 2003/12
N2 - In the present study, we investigated whether low shear (LS, 2dyn/cm 2) favors high glucose (HG, 30mM) induced nuclear factor kappa B (NF-κB) activity by regulating NO release in human aortic endothelial cells (HAEC). The results show that (i) under LS, the NF-κB activity of HAEC exposed to HG was significantly higher than HAEC in normal glucose (NG, 5.5mM) (P<0.05). In contrast, under HS, the activation of NF-κB in HAEC exposed to HG showed no significant difference compared to that of NG. (ii) The NF-κB activity induced by HG is suppressed by high shear (HS) in the absence of a NO synthase inhibitor, Nω-nitro-L-arginine methyl ester (L-NAME) but restored in its presence, while LS+HG induced NF-κB activity remains the same in the presence or absence of L-NAME. (iii) Endothelial nitric oxide synthase (eNOS) protein expression and quantitative detection of NO indicated that high shear stress significantly induced higher eNOS expression and NO production compared to low shear stress condition. Collectively, these data suggest that HS exerts a protective effect on HG induced NF-κB activity through NO mediated signaling. LS, on the other hand, may down-regulate eNOS expression resulting in reduced NO release, and thereby maintain high glucose induced NF-κB DNA-binding activity. These observations explain, in part, the mechanism by means of which hyperglycemia accelerates the focal development of atherosclerotic lesions in low shear (lesion prone) areas of the arterial tree.
AB - In the present study, we investigated whether low shear (LS, 2dyn/cm 2) favors high glucose (HG, 30mM) induced nuclear factor kappa B (NF-κB) activity by regulating NO release in human aortic endothelial cells (HAEC). The results show that (i) under LS, the NF-κB activity of HAEC exposed to HG was significantly higher than HAEC in normal glucose (NG, 5.5mM) (P<0.05). In contrast, under HS, the activation of NF-κB in HAEC exposed to HG showed no significant difference compared to that of NG. (ii) The NF-κB activity induced by HG is suppressed by high shear (HS) in the absence of a NO synthase inhibitor, Nω-nitro-L-arginine methyl ester (L-NAME) but restored in its presence, while LS+HG induced NF-κB activity remains the same in the presence or absence of L-NAME. (iii) Endothelial nitric oxide synthase (eNOS) protein expression and quantitative detection of NO indicated that high shear stress significantly induced higher eNOS expression and NO production compared to low shear stress condition. Collectively, these data suggest that HS exerts a protective effect on HG induced NF-κB activity through NO mediated signaling. LS, on the other hand, may down-regulate eNOS expression resulting in reduced NO release, and thereby maintain high glucose induced NF-κB DNA-binding activity. These observations explain, in part, the mechanism by means of which hyperglycemia accelerates the focal development of atherosclerotic lesions in low shear (lesion prone) areas of the arterial tree.
KW - Accelerated atherosclerosis
KW - Diabetes mellitus
KW - Hemodynamics
KW - eNOS
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U2 - 10.1016/j.atherosclerosis.2003.08.023
DO - 10.1016/j.atherosclerosis.2003.08.023
M3 - Article
C2 - 14644391
AN - SCOPUS:0344464839
SN - 0021-9150
VL - 171
SP - 225
EP - 234
JO - Atherosclerosis
JF - Atherosclerosis
IS - 2
ER -