High-frequency phenotypic reversion and pathogenicity of an acyclovir-resistant herpes simplex virus mutant

Anthony Griffiths, Donald M. Coen

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

A double-guanine-insertion mutation within a run of guanines in the herpes simplex virus gene encoding thymidine kinase (TK) was previously found in an acyclovir-resistant clinical isolate. This mutation was engineered into strain KOS, and stocks were generated from single plaques. Plaque autoradiography revealed that most plaques in such stocks exhibited low levels of TK activity, while ∼3% of plaques exhibited high levels of TK activity, indicating a remarkably high frequency of phenotypic reversion. This virus was able to reactivate from latency in mouse ganglia; a fraction of the reactivating virus expressed a high level of TK activity due to an additional G insertion, suggesting that the observed genetic instability contributed to pathogenicity.

Original languageEnglish (US)
Pages (from-to)2282-2286
Number of pages5
JournalJournal of Virology
Volume77
Issue number3
DOIs
StatePublished - Feb 2003
Externally publishedYes

Fingerprint

thymidine kinase
herpes simplex
Acyclovir
Thymidine Kinase
Simplexvirus
Virulence
pathogenicity
viruses
mutants
guanine
Guanine
Viruses
mutation
Insertional Mutagenesis
autoradiography
Autoradiography
Ganglia
Mutation
mice
Genes

ASJC Scopus subject areas

  • Immunology

Cite this

High-frequency phenotypic reversion and pathogenicity of an acyclovir-resistant herpes simplex virus mutant. / Griffiths, Anthony; Coen, Donald M.

In: Journal of Virology, Vol. 77, No. 3, 02.2003, p. 2282-2286.

Research output: Contribution to journalArticle

Griffiths, Anthony ; Coen, Donald M. / High-frequency phenotypic reversion and pathogenicity of an acyclovir-resistant herpes simplex virus mutant. In: Journal of Virology. 2003 ; Vol. 77, No. 3. pp. 2282-2286.
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