High-frequency phenotypic reversion and pathogenicity of an acyclovir-resistant herpes simplex virus mutant

Anthony Griffiths, Donald M. Coen

    Research output: Contribution to journalArticlepeer-review

    26 Scopus citations

    Abstract

    A double-guanine-insertion mutation within a run of guanines in the herpes simplex virus gene encoding thymidine kinase (TK) was previously found in an acyclovir-resistant clinical isolate. This mutation was engineered into strain KOS, and stocks were generated from single plaques. Plaque autoradiography revealed that most plaques in such stocks exhibited low levels of TK activity, while ∼3% of plaques exhibited high levels of TK activity, indicating a remarkably high frequency of phenotypic reversion. This virus was able to reactivate from latency in mouse ganglia; a fraction of the reactivating virus expressed a high level of TK activity due to an additional G insertion, suggesting that the observed genetic instability contributed to pathogenicity.

    Original languageEnglish (US)
    Pages (from-to)2282-2286
    Number of pages5
    JournalJournal of virology
    Volume77
    Issue number3
    DOIs
    StatePublished - Feb 2003

    ASJC Scopus subject areas

    • Microbiology
    • Immunology
    • Insect Science
    • Virology

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