High fat diet-induced animal model of age-associated obesity and osteoporosis

Ganesh V. Halade, Md M. Rahman, Paul J. Williams, Gabriel Fernandes

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

Osteoporosis and obesity remain a major public health concern through its associated fragility and fractures. Several animal models for the study of osteoporotic bone loss, such as ovariectomy (OVX) and denervation, require unique surgical skills and expensive set up. The challenging aspect of these age-associated diseases is that no single animal model exactly mimics the progression of these human-specific chronic conditions. Accordingly, to develop a simple and novel model of post menopausal bone loss with obesity, we fed either a high fat diet containing 10% corn oil (CO) or standard rodent lab chow (LC) to 12-month-old female C57Bl/6J mice for 6 months. As a result, CO fed mice exhibited increased body weight, total body fat mass, abdominal fat mass and reduced bone mineral density (BMD) in different skeletal sites measured by dual energy X-ray absorptiometry. We also observed that decreased BMD with age in CO fed obese mice was accompanied by increased bone marrow adiposity, up-regulation of peroxisome proliferator-activated receptor γ, cathepsin k and increased proinflammatory cytokines (interleukin 6 and tumor necrosis factor α) in bone marrow and splenocytes, when compared to that of LC fed mice. Therefore, this appears to be a simple, novel and convenient age-associated model of post menopausal bone loss, in conjunction with obesity, which can be used in pre-clinical drug discovery to screen new therapeutic drugs or dietary interventions for the treatment of obesity and osteoporosis in the human population.

Original languageEnglish (US)
Pages (from-to)1162-1169
Number of pages8
JournalJournal of Nutritional Biochemistry
Volume21
Issue number12
DOIs
StatePublished - Dec 2010

Fingerprint

High Fat Diet
Nutrition
Osteoporosis
Corn Oil
Bone
Animals
Animal Models
Obesity
Fats
Bone and Bones
Bone Density
Bone Marrow
Cathepsins
Obese Mice
Abdominal Fat
Peroxisome Proliferator-Activated Receptors
Photon Absorptiometry
Adiposity
Ovariectomy
Denervation

Keywords

  • Adipocytes
  • Animal model
  • Bone adiposity
  • Fat mass
  • Obesity
  • Osteoporosis
  • Proinflammatory cytokines

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

High fat diet-induced animal model of age-associated obesity and osteoporosis. / Halade, Ganesh V.; Rahman, Md M.; Williams, Paul J.; Fernandes, Gabriel.

In: Journal of Nutritional Biochemistry, Vol. 21, No. 12, 12.2010, p. 1162-1169.

Research output: Contribution to journalArticle

Halade, Ganesh V. ; Rahman, Md M. ; Williams, Paul J. ; Fernandes, Gabriel. / High fat diet-induced animal model of age-associated obesity and osteoporosis. In: Journal of Nutritional Biochemistry. 2010 ; Vol. 21, No. 12. pp. 1162-1169.
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